please helpAssume that your GFP protein was initially cleaved ... | Filo
please helpAssume that your GFP protein was initially cleaved ... | Filo
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vi-AA10ctdO
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SOLVED: Antibodies can be very useful in the expression and purification of a target protein. Explain how antibodies to the hexahistidine tag or to GFP could be used in a MEDBLAB practical. Can you think of a downstream application where it would be undesirable for a purified protein to have an affinity tag attached? How could you create a construct where the tag is removable or remove an existing tag? GFP is a relatively easy protein to express in bacteria, as it is soluble and non-toxic, even
SOLVED: Antibodies can be very useful in the expression and purifi…
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