pET-28b(+) Sequence and Map - SnapGene
pET-28b (+) is a plasmid that allows expression of recombinant proteins with a thrombin site and a 6xHis tag at the N-terminus. It has a T7 promoter and a lac operator for induction and repression, respectively. See the sequence, map, and restriction sites of pET-28b (+).Addgene: Vector Database - pET-28 b (+)
This web page provides information about pET-28 b (+), a bacterial expression vector from EMD Biosciences. It includes the vector type, size, sequence, tags, resistance, and notes, but it is …
69865 Sigma-Aldrich pET-28b(+) DNA - Novagen - EMD Millipore
pET-28b (+) DNA - Novagen. Novagen's pET-28a-c (+) vectors carry an N-terminal His•Tag ® /thrombin/T7•Tag ® configuration plus an optional C-terminal His•Tag sequence.
pET-28b (+) vector map and sequence
pET-28b (+) is a synthetic plasmid vector for protein expression in E. coli. It contains a T7 promoter, a lac operator, an RBS, a 6xHis tag, a thrombin site, and a kanamycin resistance gene.
Difference between pET-28a vector and pET-28b vector?
Sep 10, 2019 · While using pET28 series, there shouldn't be a problem when one of the cloning sites chosen are away from MCS (eg) Nde1, Nhe1, Bmt1, Nco1. In such cases, pET28a or pET28b or pET28c can be...
Improved designs for pET expression plasmids increase protein ...
May 7, 2020 · pET28a is the most popular expression plasmid on the market (described in >40,000 published articles). It contains the T7 promoter and an adjacent lac operator …
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pET-28b(+) 5.4kb. f1 O. Lac. l. T7 terminator. Blp l. His tag. Xho l Not l Hind lll Sal l Sac l EcoR l BamH l. Nhe l Thrombin Nde l His tag RBS.
pET-28a(+) Sequence and Map - SnapGene
pET-28a (+) is a plasmid that allows expression of recombinant proteins with a thrombin site and a 6xHis tag at the N-terminus. It has a T7 promoter and a lac operator for induction and …
21: pET expression system - Biology LibreTexts
Sep 23, 2024 · The pET28b vector allows the researcher to incorporate N-terminal polyhistidine (His) and T7 tags and/or a C-terminal His tag. The pET28b plasmid has been engineered such …
DNASU Plasmid | Detailed Vector Information: pET28b (+)
pET28b (+) is a bacterial expression vector with T7 promoter, kanamycin resistance and restriction enzyme cloning sites. It has a size of 5368 bp and a sequence that includes a T7 …