Engineered Cas12i2 is a versatile high-efficiency platform for
May 20, 2022 · Cas12i is a genome editing platform with compact size that fits in AAV vector with short 43-mer gRNA, absence of tracrRNA, ability to process pre-crRNA, and high specificity.
Mechanisms for target recognition and cleavage by the Cas12i ... - PubMed
Cas12i is a recently identified type V CRISPR-Cas endonuclease that predominantly cleaves the non-target strand of a double-stranded DNA substrate. This nicking activity of Cas12i could potentially be used for genome editing with high specificity.
An engineered Cas12i nuclease that is an efficient genome editing …
Mar 4, 2024 · Here, we used structure-guided rational design and protein engineering to optimize an uncharacterized Cas12i nuclease, Cas12i3. As a result, we developed Cas-SF01, a Cas12i3 variant that exhibits significantly improved gene editing activity in mammalian cells.
Mechanisms for target recognition and cleavage by the Cas12i ... - Nature
Sep 7, 2020 · Cas12i is a recently identified type V CRISPR-Cas endonuclease that predominantly cleaves the non-target strand of a double-stranded DNA substrate. This nicking activity of Cas12i could...
Mechanistic insights into the R-loop formation and cleavage in CRISPR ...
Jun 9, 2021 · Cas12i is a newly identified member of the functionally diverse type V CRISPR-Cas effectors. Although Cas12i has the potential to serve as genome-editing tool, its structural and...
Mechanisms for target recognition and cleavage by the Cas12i …
Cas12i is a recently identified type V CRISPR-Cas endonuclease that predominantly cleaves the non-target strand of a double stranded DNA substrate. The nicking activity of Cas12i can be potentially used for genome editing with high specificity.
engineered xCas12i with high activity, high ... - Oxford Academic
Nov 15, 2022 · In this study, we demonstrate that the Type V-I Cas12i system enables versatile and efficient genome editing in mammalian cells. We found a natural Cas12i variant, xCas12i, that shows high editing efficiency at TTN-PAM sites.
Engineered Cas12i2 is a versatile high-efficiency platform for ...
May 20, 2022 · Here we present an engineered Type V-I CRISPR system (Cas12i), ABR-001, which utilizes a tracr-less guide RNA. The compact Cas12i effector is capable of self-processing pre-crRNA and cleaving dsDNA targets, which facilitates versatile delivery options and multiplexing, respectively.
Functionally diverse type V CRISPR-Cas systems | Science - AAAS
Dec 6, 2018 · Cas12i exhibits markedly different efficiencies of CRISPR RNA spacer complementary and noncomplementary strand cleavage resulting in predominant dsDNA nicking. Cas12g is an RNA-guided ribonuclease (RNase) with …
Cas12i exhibits markedly different efficiencies of CRISPR RNA spacer complementary and noncomplementary strand cleavage resulting in predominant dsDNA nicking. Cas12g is an RNA-guided ribonuclease (RNase) with collateral RNase and single-strand DNase activities.
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