- Here is a protocol for staining cells with DAPI1234:
- Wash the cells 1–3 times in PBS as needed.
- Add sufficient 300 nM DAPI stain solution to cover the cells.
- Incubate for 1–5 minutes, protected from light.
- Remove the stain solution.
- Wash the cells 2–3 times in PBS.
- Image the cells.
Learn more:✕This summary was generated using AI based on multiple online sources. To view the original source information, use the "Learn more" links.1. Wash the cells 1–3 times in PBS as needed. 2. Add sufficient 300 nM DAPI stain solution to cover the cells. 3. Incubate for 1–5 minutes, protected from light. 4. Remove the stain solution. 5. Wash the cells 2–3 times in PBS. 6. Image the cells. DAPI is a known mutagen and should be handled with care.
www.thermofisher.com/us/en/home/references/prot…In brief, cells in suspension are spun and resuspended in a solution of 10µg/ml 4,6-diamidino-2-phenylindole (DAPI) and 0.1% nonidet P-40 detergent in a Tris buffered saline. The suspension is triturated with a 26 gauge needle and analyzed on the cytometer, with ultraviolet or violet excitation and DAPI emission collected at >450nm.www.pathology.washington.edu/research/labs/rabi…Recommended Assay Procedures Staining of Live Cells for Viability Analysis by Flow Cytometry 1. Obtain a single cell suspension. 2. Resuspend cells in BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) or 1× Dulbecco's Phosphate Buffered Saline (DPBS) containing 0.05-0.2 μg/mL DAPI.www.bdbiosciences.com/en-us/products/reagents/f…Staining Protocols. Live cell staining. Below we provide two protocols for staining live cells with DAPI or Hoechst. Staining by medium exchange results in uniform exposure of cells to probe. However, for some cell types, morphology or viability may be affected by medium exchange. In addition, floating dead cells may be lost during medium removal, and suspension cells must be collected by ...
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DAPI Protocol for Fluorescence Imaging - Thermo …
Step-by-step protocol for the use of DAPI (4′,6-diamidino-2-phenylindole) for nuclear acid (nuclear) staining in fluorescence microscopy.
Other content from thermofisher.comHoechst & DAPI Staining Protocols – Cell Staining with ... - Biotium
See more on biotium.comHoechst and DAPI are popular blue fluorescent, nuclear-specific dyes that can be used to stain live or fixed cells. Both DAPI and Hoechst are minor-groove binding dyes with a preference for A/T-rich regions of DNA over G/C-rich DNA. The dyes have minimal fluorescence in solution, but become brightly fluorescent upon bindi…- Estimated Reading Time: 7 mins
DAPI Counterstaining Protocols | Thermo Fisher Scientific - US
DAPI is a popular nuclear counterstain for use in multicolor fluorescent techniques. Its blue fluorescence stands out in vivid contrast to green, yellow, or red fluorescent probes of other …
Labeling Nuclear DNA Using DAPI - CSH Protocols
This protocol describes the use of DAPI to label nuclear DNA of cells grown in culture. Autofluorescence from endogenous cellular molecules such as the reduced forms of …
DAPI is a popular nuclear counterstain for use in multicolor fluorescent techniques. Its blue fluorescence stands out in vivid contrast to green, yellow, or red fluorescent probes of other …
DAPI Staining Protocol - McGill University
DAPI Staining Protocol. Prepare the following solutions: (To determine the amount needed for your experiment, please note that one tube of Solution 1 will yield a total of 100mL of working …
Cell assay, fix and stain (DAPI, Phalloidin)
This is the DRSC's basic protocol for fixation with formaldehyde and staining with DAPI and/or phalloidin. It is also appropriate for fixation prior to immunostaining, although some antibodies, …
The counterstaining protocols are compatible with a wide range of cytological labeling techniques, direct or indirect antibody-based detection methods, mRNA in situ hybridization or …
What is DAPI staining used for? - SyronOptics
DAPI, or 4',6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to the A-T rich regions of DNA. When bound to.
Labeling nuclear DNA using DAPI - PubMed
PMID: 21205856. DOI: 10.1101/pdb.prot5556. Abstract. A number of fluorescent stains are available that label DNA and allow easy visualization of the nucleus in interphase cells and …