- Here is a protocol for staining cells with DAPI1234:
- Wash the cells 1–3 times in PBS as needed.
- Add sufficient 300 nM DAPI stain solution to cover the cells.
- Incubate for 1–5 minutes, protected from light.
- Remove the stain solution.
- Wash the cells 2–3 times in PBS.
- Image the cells.
Learn more:✕This summary was generated using AI based on multiple online sources. To view the original source information, use the "Learn more" links.1. Wash the cells 1–3 times in PBS as needed. 2. Add sufficient 300 nM DAPI stain solution to cover the cells. 3. Incubate for 1–5 minutes, protected from light. 4. Remove the stain solution. 5. Wash the cells 2–3 times in PBS. 6. Image the cells. DAPI is a known mutagen and should be handled with care.
www.thermofisher.com/us/en/home/references/prot…In brief, cells in suspension are spun and resuspended in a solution of 10µg/ml 4,6-diamidino-2-phenylindole (DAPI) and 0.1% nonidet P-40 detergent in a Tris buffered saline. The suspension is triturated with a 26 gauge needle and analyzed on the cytometer, with ultraviolet or violet excitation and DAPI emission collected at >450nm.www.pathology.washington.edu/research/labs/rabi…Recommended Assay Procedures Staining of Live Cells for Viability Analysis by Flow Cytometry 1. Obtain a single cell suspension. 2. Resuspend cells in BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) or 1× Dulbecco's Phosphate Buffered Saline (DPBS) containing 0.05-0.2 μg/mL DAPI.www.bdbiosciences.com/en-us/products/reagents/f…Staining Protocols. Live cell staining. Below we provide two protocols for staining live cells with DAPI or Hoechst. Staining by medium exchange results in uniform exposure of cells to probe. However, for some cell types, morphology or viability may be affected by medium exchange. In addition, floating dead cells may be lost during medium removal, and suspension cells must be collected by ...
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