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  1. Nature
    https://www.nature.com/articles/s41594-023-01041-4

    Collateral lethality between HDAC1 and HDAC2 exploits cancer

    • Transcriptional co-regulators have been widely pursued as targets for disrupting oncogenic gene regulatory programs. However, many proteins in this target class are universally essential for cell survival, which li… See more

    Main

    Currently, four HDAC inhibitors are approved for use as anti-cancer agents in humans1, but … See more

    Nature
    Results

    Lineage-selective HDAC1 and HDAC2 cancer dependencies
    To systematically evaluate the requirement for individual class I HDACs in cancer, we extracted s… See more

    Nature
    Discussion

    This study applied a generalizable framework for identifying synthetic lethal genetic interactions. We identified HDAC1/2 as a reciprocal synthetic lethality caused by … See more

    Nature
    Methods

    Cell culture and lentivirus production
    BE(2)-C (DMEM supplemented with 10% fetal bovine serum (FBS) and Gibco antibiotic–antimycotic), SK-N-AS (DMEM supplement… See more

    Nature
    Data availability

    For availability of the data from DepMap and cBioportal see ‘Analyses of datasets from DepMap’ and ‘Analyses of datasets from cBioportal.’ All raw next-generation sequencin… See more

    Nature
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    Table of Contents
    Main
    Results
    Discussion
    Methods
    Data availability
    What does HDAC1 do?
     
  1. Studies of sgRNA HDAC1
    National Center for Biotechnology Information
    Histone Deacetylases (HDACs): Evolution, Specificity, Role in Transcriptional Complexes, and Pharmacological Actionability
    Histone deacetylases (HDACs) are evolutionary conserved enzymes which operate by removing acetyl groups from histones and other protein regulatory factors, with functional consequences on chromatin remodeling and gene expression profiles. We provide here a review on the recent knowledge accrued on t...
    Author: Giorgio Milazz…
    Published: 2020/05
    Publication: Genes (B…
    National Center for Biotechnology Information
    Histone hyperacetylation disrupts core gene regulatory architecture in rhabdomyosarcoma
    There are more than 1,500 transcription factors (TFs) encoded in the human genome1. Some TFs are used across all human cell types (such as the General Transcription Factors2), while many TFs are restricted to a particular time and place in development3,4. In a given cell type, a few core regulatory ...
    Author: Berkley E. Gry…
    Published: 2019/12
    DOI: 10.1038/s41588-0…
    National Center for Biotechnology Information
    HDAC1 and HDAC2 Double Knockout Triggers Cell Apoptosis in Advanced Thyroid Cancer
    Anaplastic thyroid carcinoma (ATC) and squamous thyroid carcinoma (STC) are both rare and advanced thyroid malignancies with a very poor prognosis and an average median survival time of 5 months and less than 20% of affected patients are alive 1 year after diagnosis. The clinical management of both ...
    Author: Ching-Ling Lin…
    Published: 2019/01
    Publication: Int J Mol …
    ScienceDirect
    Polycistronic tRNA and CRISPR guide-RNA enables highly efficient multiplexed genome engineering in human cells
    CRISPR/Cas9 has been widely used for genomic editing in many organisms. Many human diseases are caused by multiple mutations. The CRISPR/Cas9 system provides a potential tool to introduce multiple mutations in a genome. To mimic complicated genomic variants in human diseases, such as multiple gene d...
    Published: 2017/01/22
    Cited By: 0
    Keywords: Genome e…
    PubMed
    Inhibition of histone deacetylase 1 (HDAC1) and HDAC2 enhances CRISPR/Cas9 genome editing
    Despite the rapid development of CRISPR/Cas9-mediated gene editing technology, the gene editing potential of CRISPR/Cas9 is hampered by low efficiency, especially for clinical applications. One of the major challenges is that chromatin compaction inevitably limits the Cas9 protein access to the targ...
    Author: Bin Liu
    Published: 2020/01/24
    Keywords: National In…
    National Center for Biotechnology Information
    Class I histone deacetylases (HDAC) critically contribute to Ewing sarcoma pathogenesis
    Background Histone acetylation and deacetylation seem processes involved in the pathogenesis of Ewing sarcoma (EwS). Here histone deacetylases (HDAC) class I were investigated. Methods Their role was determined using different inhibitors including TSA, Romidepsin, Entinostat and PCI-34051 as ...
    Author: Oxana Schmid…
    Published: 2021
    Publication: J Exp Cli…
  2. Nature
    https://www.nature.com/articles/s41421-023-00573-9

    HDAC1/2/3 are major histone desuccinylases critical for promoter ...

  3. Nature
    https://www.nature.com/articles/s41588-01…

    Histone hyperacetylation disrupts core gene regulatory …

    WEBNov 29, 2019 · Triple inhibition of HDAC1, HDAC2 and HDAC3 with Entinostat increases histone acetylation not only at H3K27 but also at H2BK5 and H4K16, and to a lesser extent at H3K36 (Fig. 4b).

  4. BMC Cancer
    https://bmccancer.biomedcentral.com/articles/10...

    Pooled CRISPR screening in pancreatic cancer cells implicates co ...

  5. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288346

    Histone Deacetylases (HDACs): Evolution, Specificity, Role in ...

  6. BMC Cancer
    https://bmccancer.biomedcentral.com/articles/10...

    Genomic analysis reveals HDAC1 regulates clinically relevant ...

  7. People also ask
    Does top HDAC1 sgRNA cause transcriptional changes?
    Our top HDAC1 sgRNA induced greater than 10-fold overexpression of HDAC1. Overexpression of this transcriptional regulator also produced several weaker transcriptional changes that we hypothesize to be downstream of HDAC1-mediated transcriptional regulation (Fig. 4 B).

    Pooled CRISPR screening in pancreatic cancer cells implicates co-repres…

    bmccancer.biomedcentral.com
    Does siRNA reduce HDAC1 and HDAC2?
    We confirmed by WB analysis that the siRNA treatment resulted in a more than 70% reduction of HDAC1, HDAC2, and HDAC3 proteins in all three cell lines (Fig. 3c). Notably, knockdown (KD) of HDAC1/2/3 by siRNA also resulted in a similar elevation of histone succinylation (Fig. 3c).

    HDAC1/2/3 are major histone desuccinylases critical for promoter desuc…

    nature.com
    What does HDAC1 do?
    HDAC1 regulates the acetylation of histone and non-histone proteins to modulate gene expression and its overexpression has been associated with progression, metastasis, and patient prognosis in many cancer types including gastric, breast, colon, and prostate cancers .

    Genomic analysis reveals HDAC1 regulates clinically relevant transcripti…

    bmccancer.biomedcentral.com
    Is hdac1/2/3 a major histone desuccinylase in mammalian cells?
    Notably, knockdown (KD) of HDAC1/2/3 by siRNA also resulted in a similar elevation of histone succinylation (Fig. 3c). These results therefore demonstrated that HDAC1/2/3 have redundant role in histone desuccinylation and likely represent the major histone desuccinylases in mammalian cells.

    HDAC1/2/3 are major histone desuccinylases critical for promoter desuc…

    nature.com
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  8. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6886578

    Histone hyperacetylation disrupts core gene regulatory …

  9. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359659

    HDAC1 and HDAC2 Double Knockout Triggers Cell Apoptosis in …

  10. ScienceDirect
    https://www.sciencedirect.com/science/article/pii/S0006291X16320034

    Polycistronic tRNA and CRISPR guide-RNA enables highly efficient ...

  11. American Association for Cancer Research
    https://aacrjournals.org/clincancerres/arti…

    Targeting DNA Damage Repair Functions of Two …

    WEBApr 15, 2021 · AML cells are addicted to nicotinamide phosphoribosyltransferase (NAMPT)-mediated salvage pathway for the biosynthesis of NAD + (3, 4), which serves as a coenzyme for redox …