para-Nitrophenylphosphate - Wikipedia
para-Nitrophenylphosphate (pNPP) is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays. [1] Phosphatases catalyze the hydrolysis of pNPP liberating inorganic phosphate and the conjugate base of para-nitrophenol (pNP).
PNPP Substrates - Thermo Fisher Scientific
PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.
p-Nitrophenyl Phosphate (PNPP) - NEB
p -Nitrophenyl Phosphate (PNPP) is a non-proteinaceous, non-specific substrate used to assay protein, alkaline and acid phosphatases. Can be used for quick analysis of the protein phosphatase activity under any conditions.
pNPP (p-Nitrophenyl Phosphate) | Sigma-Aldrich
pNPP (p-Nitrophenyl Phosphate); find Sigma-Aldrich-20106 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.
p-Nitrophenyl phosphate | C6H4NO6P-2 | CID 4686862 - PubChem
p-Nitrophenyl phosphate | C6H4NO6P-2 | CID 4686862 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
pNPP Phosphatase Assay Kit, MAK461, 500 Tests, Sigma-Aldrich
The pNPP Phosphatase Assay kit involves adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37 °C.
ELISA Enzyme Substrates | Thermo Fisher Scientific - US
PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. PNPP produces a yellow water-soluble reaction product that absorbs light at 405 nm.
p -Nitrophenyl Phosphate Liquid Substrate System
The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays. [1]
Protein tyrosine phosphatase: enzymatic assays - ScienceDirect
Jan 1, 2005 · pNPP is a colorless substrate that hydrolyzes to p -nitrophenol. The phenolate ion (p Ka of 7.2) is soluble and its bright yellow color can be monitored by absorbance at 405 nm. Because of its p Ka, the spectroscopic properties of the ion are strongest at alkaline pH.
pNPP-Na hexahydrate, Alkaline phosphatase substrate - Abcam
pNPP-Na hexahydrate, Alkaline phosphatase substrate. MW 371.15 g/mol, Purity >98%. Chromogenic substrate for acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm. Can be used to perform kinetic and endpoint determinations.