pET-28b(+) Sequence and Map - SnapGene
Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28a (+) or pET-28c (+). Sequence Author: MilliporeSigma (Novagen) Open in SnapGene. Try SnapGene for Free. Download Plasmid.
Addgene: Vector Database - pET-28 b (+)
pET-28 b (+) Vector Database. Welcome to Vector Database! Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources. This vector is NOT available from Addgene and the database is no longer actively maintained. Search vector database.
pET-28b(+) vector map and sequence
Provide pET-28b (+) vector/plasmid map, full length sequence, antibiotic resistance, size and other information.
pET-28b(+) DNA - Novagen | 69865 - Merck
pET-28b (+) DNA - Novagen Novagen's pET-28a-c (+) vectors carry an N-terminal His•Tag®/thrombin/T7•Tag® configuration plus an optional C-terminal His•Tag sequence. - Find MSDS or SDS, a COA, data sheets and more information.
Improved designs for pET expression plasmids increase protein
May 7, 2020 · Patrick Shilling et al. increase the protein production yield from the pET28a expression plasmid by modifying the genetic modules that control transcription and translation initiation. These ...
DNASU Plasmid | Detailed Vector Information: pET28b (+)
Vector Name: pET28b (+) Synonyms: pET, pET28, pET28b, pET28b+, pET28b-plus. Sequencing Primer: Forward: T7. Reverse: T7 terminator. Description: Bacterial expression vector with T7 promoter; kanamycin resistance; restriction enzyme cloning.
pET-28b(+) - EcoliWiki
Aug 25, 2011 · The pET-28b (+) vector carries an N-terminal His•Tag/thrombin/T7•Tag configuration in addition to an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map. The T7 expression region is reversed on the circular map due to the sequence numbering from the pBR322 convention.
pET-28b(+) DNA - Novagen - MilliporeSigma
pET-28b (+) DNA - Novagen. Novagen′s pET-28a-c (+) vectors carry an N-terminal His•Tag®/thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence. Pricing and Inventory is not available.
pET-28b(+) DNA - Novagen - MilliporeSigma
pET-28b (+) DNA - Novagen. Novagen′s pET-28a-c (+) vectors carry an N-terminal His•Tag /thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence.
Improved designs for pET expression plasmids increase protein ...
May 7, 2020 · A statistically significant difference of p < 0.001 relative to pET28a (two-tailed Student’s t test) is denoted by ***. In this study, we have identified design flaws in the pET series of expression plasmids, which limit protein production yields.