Solved 11. The lacZ gene, responsible for lactose metabolism
The lacZ gene, responsible for lactose metabolism | Chegg.com. 11. The lacZ gene, responsible for lactose metabolism is an inducible gene regulated by a repressor protein. Answer the following questions regarding how this gene is regulated (3 pts each) a. What is the default setting in reference to the expression of lacZ?
Solved A. Explain how colony color can be used as evidence - Chegg
A. Explain how colony color can be used as evidence of the state of the lacZ gene in the bacteria. B. Which plates show evidence of the lacZ gene having been cut by Case? C. Of the plates that show evidence of the lacZ gene having been cut, which also show evidence of the DNA cut having been repaired? Note that repairing DNA does not mean ...
Solved lacZ CRISPR Gene Editing Laboratory In this activity - Chegg
Conveniently, β-gal also breaks down the. lacZ CRISPR Gene Editing Laboratory. In this activity you will use CRISPR-Cas9 to cut the bacterial chromosome DNA within the lacZ. gene, which codes for the enzyme β-galactosidase (β-gal). The lacZ gene is part of the lac operon, a. collection of genes that allows bacteria to use lactose, a milk ...
Pre-Laboratory Questions Table 1. Predicted Results | Chegg.com
Biology. Biology questions and answers. Pre-Laboratory Questions Table 1. Predicted Results of Multiplex PCR. Retrieve your analysis and answers from the lacZ CRISPR Gene Editing Laboratory Activity to answer the following questions. A. In Table 1, fill in "colony appearance" and "lacZ gene status" with your current conclusions about the lacZ ...
Solved Operons are controlled at the level of transcription ... - Chegg
Here’s the best way to solve it. Answer - IT BINDS TO LAC OPERATOR The lac repressor protein bin …. Operons are controlled at the level of transcription. A regulator molecule can be transcribed and translated to block the operator so that the lacZ gene protein is only made when lactose is present in the environment.
Solved (SELECT ALL THAT APPLY) If an experimenter moves - Chegg
A.) RNA polymerase will only transcribe the gene for permease. B.) Both genes will be expressed normally. C.) RNA polymerase will only transcribe the gene. (SELECT ALL THAT APPLY) If an experimenter moves the promoter of the lac operon to the region between the beta-galactosidase (lacZ) gene and the permease (lacY) gene, which of the following ...
Solved Explain why there is a part of the LacZ gene in - Chegg
To get started, identify how the LacZ gene functions as a selectable marker in the pET32a (+) vector during gene cloning or recombinant DNA technology. Answer: LacZ gene is used as selectable marker in the vector (pET 32 a)during gene cloning or rDNA technology i.e. it is used to select recombinants from non-recombinants in gene cloning ...
Solved CRISPR LAB Of the plates that show evidence of the - Chegg
Here’s how to approach this question. Assess which plates exhibit white colonies, indicating the lacZ gene was disrupted by the insertion of foreign DNA, showing evidence of a cut. The question is in context of LacZ screening: Background: lacZ gene encodes for b-galactosidase protein which cleaves X-gal a colourless analog of lactose.
Solved Suppose a scientist wants to clone the gene for human
Question: Suppose a scientist wants to clone the gene for human insulin. He uses a plasmid that contains the lacZ gene, an origin of replication, and an ampicillin resistant gene. He inserts the human insulin gene into a restriction site located within the lacZ gene. Then he transforms this plasmid into bacteria without a functional lacZ gene ...
Solved A. In Table 1, fill in “colony appearance” and
A. In Table 1, fill in “colony appearance” and “lacZ gene status” with your current conclusions about the lacZ gene in the bacteria on the IX/ARA starter plate, plate C, and plate D from the lacZ CRISPR Gene Editing Laboratory. If there were colonies with different appearances on the same plate, include them all in your ...