The Biotechnology Revolution: PCR and Cloning Expressed Genes
WEBJun 27, 1970 · The cloning of expressed genes and the polymerase chain reaction (PCR), two biotechnological breakthroughs of the 1970s and 1980s, continue to play significant roles in science today. Both ...
DNA cloning by homologous recombination in Escherichia coli
WEBThe cloning of foreign DNA in Escherichia coli episomes is a cornerstone of molecular biology. The pioneering work in the early 1970s, using DNA ligases to paste DNA into episomal vectors, is ...
Cloning - Latest research and news | Nature
WEBFeb 29, 2024 · Cloning is a method that is used to produce genetically identical copies of pieces of DNA, cells or organisms. Cloning methods include: molecular cloning, which makes copies of pieces of DNA ...
recombinant DNA technology / DNA cloning; gene cloning; …
WEBIn this host cell, the customized recombined DNA sequence can be copied or translated. This page has been archived and is no longer updated recombinant DNA technology / DNA cloning; gene cloning ...
A unified multi-kingdom Golden Gate cloning platform
WEBJul 12, 2019 · Assembling composite DNA modules from custom DNA parts has become routine due to recent technological breakthroughs such as Golden Gate modular cloning. Using Golden Gate, one can efficiently ...
Harnessing homologous recombination in vitro to generate
WEBFeb 11, 2007 · Abstract. We describe a new cloning method, sequence and ligation–independent cloning (SLIC), which allows the assembly of multiple DNA fragments in a single reaction using in vitro homologous ...
Human cloning: can it be made safe? | Nature Reviews Genetics
WEBNov 1, 2003 · Human reproductive cloning is unethical, but the production of cells from cloned embryos could offer many potential benefits. ... DNA methylation. DNA methylation is important in the regulation of ...
Cloning and mutagenesis: tinkering with the order of things
WEBNature Methods 4, 455–461 (2007) Cite this article. Engineering the replication of target DNA through cloning, or changing its genetic code through mutations, are detail-oriented processes whose ...
IVA cloning: A single-tube universal cloning system exploiting ...
WEBJun 6, 2016 · Molecular cloning is at the heart of biomedical and biotechnological research, fundamental to protein structure-function studies, protein engineering and synthetic biology 1,2,3.Since the advent ...
Precise DNA cleavage using CRISPR-SpRYgests - Nature
WEBOct 6, 2022 · Methods for in vitro DNA cleavage and molecular cloning remain unable to precisely cleave DNA directly adjacent to bases of interest. Restriction enzymes (REs) must bind specific motifs, whereas ...
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