What effect does vortexing have on a fluid sample that simple ...
The vortex not only provides a different profile of mixing, but has the power to mix viscous fluids that would otherwise remain virtually unmixed (glycerol stocks come to mind). Vortex shakers …
Why is it advised to avoid bubble formation during mixing?
Feb 4, 2016 · $\begingroup$ I want to point out about undergrad labs: People vortex SDS mixtures and small volumes, and advertently the pipettes can't get everything out of the tube …
What is the most critical step of plasmid purification?
Sep 17, 2014 · When mixing, it's only ok to vortex the cell pellet prior to lysis, after adding lysis buffer, ONLY MIX BY INVERTING THE TUBE. Vortexing after this point can shear DNA. If you …
biochemistry - Forgot to vortex antibody before staining - Biology ...
Jun 27, 2016 · $\begingroup$ i was taught never to vortex proteins that i wanted to do something useful, but the even mixing (by pipetting or inversion or extended nutation or whatever) of a …
Why is it sometimes difficult to resuspend E. coli in P1?
$\begingroup$ When I do giga preps, I have to weigh the pellets and put 7g of cells into a 1L pyrex bottle, then add the P1 and a small stir bar that helps beat the pellet apart with vigorous …
PCR Mastermix Preparation - Biology Stack Exchange
Feb 5, 2019 · There are four things you can change depending on how much DNA you need to insert into the cloning vector: (i) amount of PCR reaction mixtures ("how many tubes")
Why is DNA polymerase added at the end of PCR reaction?
Nov 14, 2018 · PCR reaction is used to amplify DNA fragment. Each reaction requires a DNA template, buffer, dNTP mix and a unique pair of primers, one ''forward'' primer and one …
How to prepare sample for SDS PAGE? - Biology Stack Exchange
Nov 13, 2014 · I usually take my protein sample 0.8ml and sample buffer(2X) 0.2ml for my sample preparation in SDS PAGE. Am I using correct proportion? my protein sample concentration is …
Why do you have to submerge your gel in buffer before adding DNA
Jan 13, 2023 · This has a very simple reason: Adding the buffer later poses the risk to wash out your sample from the well as well as mixing samples. There is another reason: It makes …
Meaning of units in ELISA based tests? - Biology Stack Exchange
Jul 14, 2019 · A human serum pool negative for anti-dsDNA IgG antibodies (DNANe) was prepared by mixing equal parts of serum from 10 healthy persons who had a negative CLIFT …