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  1. Primers avoid amplifying nonspecific sequences
    The touchdown polymerase chain reaction or touchdown style polymerase chain reaction is a method of polymerase chain reaction by which primers avoid amplifying nonspecific sequences. The annealing temperature during a polymerase chain reaction determines the specificity of primer annealing.

    Touchdown polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Touchdown_polymerase_chain_reaction
    en.wikipedia.org/wiki/Touchdown_polymerase_chain_reaction
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    What is touchdown PCR?
    Anyone you share the following link with will be able to read this content: Provided by the Springer Nature SharedIt content-sharing initiative Touchdown (TD) PCR offers a simple and rapid means to optimize PCRs, increasing specificity, sensitivity and yield, without the need for lengthy optimizations and/or the redesigning of primers.

    Touchdown PCR for increased specificity and sensitivity in PCR

    nature.com
    Is touchdown PCR a good amplification technique?
    Touchdown PCR is a very useful technique for improving PCR amplification specificity and sensitivity. However, using this technique in the lab can be trickier than it might seem at first. So in this article, I’ll provide a primer on touchdown PCR and give you 5 key tips and references for perfecting it.

    Touchdown PCR: Easy Intro Plus 5 Expert Tips - Bitesize Bio

    bitesizebio.com
    What is annealing temperature in Touchdown PCR?
    In touchdown PCR, the annealing temperature of the first few cycles is set to be a few degrees higher than the highest melting temperature (T m) of the primers [1,2]. Higher temperatures help destabilize the formation of primer-dimers and nonspecific primer-template complexes, thus minimizing undesirable amplification.

    PCR Methods—Top Ten Strategies - Thermo Fisher Scientific - US

    thermofisher.com
    What is a touchdown polymerase chain reaction?
    The touchdown polymerase chain reaction or touchdown style polymerase chain reaction is a method of polymerase chain reaction by which primers avoid amplifying nonspecific sequences. The annealing temperature during a polymerase chain reaction determines the specificity of primer annealing.

    Touchdown polymerase chain reaction - Wikipedia

    en.wikipedia.org
    How does touchdown PCR increase specificity?
    Touchdown PCR increases specificity by using reaction conditions that gradually reduce the annealing temperature. The initial annealing temperature is set to several degrees above the estimated Tm of the primers.

    What is touchdown PCR (TD-PCR) and when would I need to use it? - Ta…

    help.takarabio.com
    Does 'touchdown' polymerase chain reaction increase sensitivity and specificity in mantle cell lymphoma?
    Duckworth, A.W. & Rule, S.A. The use of 'touchdown' polymerase chain reaction increases the sensitivity and specificity of t (11;14) (q13;q32) detection in patients with mantle cell lymphoma. Br. J. Haematol. 121, 952–953 (2003).

    Touchdown PCR for increased specificity and sensitivity in PCR

    nature.com
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  3. Nature
    https://www.nature.com/articles/nprot.2008.133

    Touchdown PCR for increased specificity and sensitivity in PCR

  5. NEB
    https://www.neb.com/en/faqs/0001/01/01/what-is-touchdown-pcr

    What is touchdown PCR? - NEB

    Tags:
    Touchdown Pcr Annealing Temperature
    Touchdown Pcr Method
  6. Bitesize Bio
    https://bitesizebio.com/2203

    Touchdown PCR: Easy Intro Plus 5 Expert Tips - Bitesize …

    Mar 6, 2023 · Learn what touchdown PCR is, how it works, and how to perform it successfully. Touchdown PCR is a modification of PCR that reduces non-specific amplification by gradually lowering the annealing temperature …

    Tags:
    Laboratory Techniques in Biology
    Polymerase Chain Reaction
    PCR Amplification
  7. PubMed
    https://pubmed.ncbi.nlm.nih.gov/29717053

    Touchdown Polymerase Chain Reaction (PCR) - PubMed

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    Touchdown Pcr Annealing Temperature
    Touchdown polymerase chain reaction
  8. Thermo Fisher Scientific
    https://www.thermofisher.com/us/en/h…

    PCR Methods—Top Ten Strategies - Thermo Fisher …

    Learn about different PCR methods and their benefits for specificity, yield, time, and convenience. Touchdown PCR is one of the methods that starts with a higher-than-optimal annealing temperature and gradually lowers it to …

    Tags:
    Touchdown Pcr Annealing Temperature
    Laboratory Techniques in Biology
  9. takarabio.com
    https://help.takarabio.com/36168/kb/article/110050

    What is touchdown PCR (TD-PCR) and when would I need to use it?

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    Touchdown polymerase chain reaction
    Touchdown Pcr Annealing Temperature
  10. 知乎专栏
    https://zhuanlan.zhihu.com/p/632238276

    降落PCR（touchdown PCR） - 知乎专栏

    May 25, 2023 · 降落PCR是一种提高PCR特异性的方法，通过逐步降低退火温度，使特异产物积累并竞争优势，最后以较低的退火温度进行扩增。本文介绍了降落PCR的基本原理、优点、特点和注意事项，以及与温度梯度PCR的区别和适 …

  11. Cold Spring Harbor Protocols
    https://cshprotocols.cshlp.org/content/2018/5/pdb.prot095133.short

    Touchdown Polymerase Chain Reaction (PCR) - CSH Protocols

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    Touchdown polymerase chain reaction
    Touchdown Pcr Annealing Temperature
  12. PubMed
    https://pubmed.ncbi.nlm.nih.gov/18772872

    Touchdown PCR for increased specificity and sensitivity in PCR ...

    Tags:
    Laboratory Techniques in Biology
    Increase Pcr Specificity