Thiazole Orange Dye content 90 107091-89-4 - MilliporeSigma
Thiazole Orange Dye content ~90 %; CAS Number: 107091-89-4; Synonyms: 1-Methyl-4- [ (3-methyl-2 (3H)-benzothiazolylidene)methyl]quinolinium p-tosylate at Sigma-Aldrich.
Thiazole Orange - an overview | ScienceDirect Topics
Apr 6, 2012 · Thiazole Orange is a fluorescent dye commonly used in chemistry as a 'light-up probe' in assays such as real-time PCR. It exhibits a significant increase in fluorescence intensity when bound to a complementary nucleic acid, making it a valuable tool for monitoring amplification reactions.
Thiazole Orange - an overview | ScienceDirect Topics
The example of a firmly established dye that has found numerous applications as a DNA staining agent is thiazole orange (TO).
Ultrafast Excited-State Dynamics of Thiazole Orange
Jan 1, 2022 · Thiazole orange (TO), an asymmetric cyanine dye, has been widely used in biomolecular detection and imaging of DNA/ RNA in gels, due to its unique fluorogenic behavior: fluorescence of free dye in aqueous solution is very weak but emission can be significantly enhanced in nucleic-acid-bound dye.
Design of thiazole orange oligonucleotide probes for detection …
Apr 17, 2019 · Thiazole orange (TO) is an unsymmetrical cyanine dye which can be conjugated to oligonucleotides (ONs) to create fluorogenic hybridisation probes. 1–3 TO becomes highly fluorescent upon binding to nucleic acids due to restriction of rotation about the methine bridge, hence TO–ON conjugates have been used for the detection of target nucleic ...
Thiazole orange as an everyday replacement for ethidium bromide and ...
We show that thiazole orange, the parent compound of SYBR Safe, is an excellent, simple, and inexpensive alternative to these dyes. It is excitable with safe blue light or UV light, with DNA detection limits in agarose gels similar to ethidium bromide and SYBR Safe (1-2 ng/lane).
Comprehensive Study of Thiazole-Orange-Based DNA Dyes
May 7, 2024 · This article presents a comprehensive study of six Thiazole-Orange-based fluorescent DNA-binding dyes: SYBR Safe, SYBR Green, Pico Green, SYTO-16, SYTO-9, and the benzothiazole-based analogue (TOPhBu) of the latter.
Thiazole orange | C26H24N2O3S2 | CID 6438345 - PubChem
Thiazole orange is a cyanine dye comprising the thiazole orange cation [1-methyl-4-[(3-methyl-1,3-benzothiazol-2(3H)-ylidene)methyl]quinolinium] with the p-tosylate counterion. It has a role as a fluorochrome.
Broad Applications of Thiazole Orange in Fluorescent Sensing of ...
May 10, 2021 · Over the past 30 years, thiazole orange (TO) has garnered great attention due to its low fluorescence background signal and remarkable 'turn-on' fluorescence response, being controlled only by its intramolecular torsional movement.
Analysis of the mechanism of damage produced by thiazole orange ...
Apr 17, 2020 · Thiazole Orange (TO), a photosensitizing nucleic acid dye, was previously shown to photoinactivate several types of bacterial and viral pathogens in RBC suspensions without adverse effects on function.