Real-Time PCR | Thermo Fisher Scientific - US
Learn about real-time PCR (qPCR), a technique for sensitive and specific detection and quantification of nucleic acid targets. Find products, applications, data analysis, and workflow solutions for qPCR research and bioanalysis.
Applied Biosystems Real-Time PCR Instruments - Thermo Fisher Scientific
Real-time PCR (qPCR) plastics combability guide. Select your real-time PCR (qPCR) instrument from the drop-down menu, and easily find the plates, optical caps, and optical seals compatible with your instrument. Calibration and Verification Kits.
Real-Time PCR (qPCR) Basics - Thermo Fisher Scientific - US
Learn the principles, applications, and best practices of real-time PCR (qPCR), a quantitative method that combines PCR amplification and detection. Explore videos, webinars, articles, and resources from the Taq Team experts.
Efficiency of Real-Time PCR | Thermo Fisher Scientific - US
Learn about qPCR efficiency, how to assess geometric efficiency, the visual assessment method and possible disadvantages of using standard curves. Discover how to perform the ΔΔCt method and its benefits.
The family of most-cited Applied BiosystemsTM qPCR solutions delivers the caliber of results you need, faster and easier than you could have imagined. Free yourself from extra steps, preventable rework, and other ineficiencies so you can focus on making meaningful discoveries.
Compare different models of QuantStudio real-time PCR and digital PCR systems for your research needs. Find out the features, benefits, applications, and prices of each system.
Learn how to optimize and troubleshoot your QPCR assay using best practice methodologies and tips from Thermo Fisher Scientific. Find out how to test and improve your template quality, primer design, thermal protocol, and more.
TaqMan® multiplex qPCR Accurate, sensitive, and as efficient as traditional qPCR In this application note, we show: • A new multiplexing solution with probes having ABY ® and JUN® reporter dyes and a QSY ® nonfluorescent quencher • An optimized TaqMan ® Multiplex Master Mix that can be used for genotyping and gene expression analysis
Essentials of Real-Time PCR | Thermo Fisher Scientific - US
Learn about real-time PCR (qPCR), a technique that monitors the progress of PCR as it occurs and enables quantitation of DNA and RNA. Compare TaqMan and SYBR Green I dye chemistries, and explore assay types and applications.
Important Parameters of Quantitative PCR (qPCR) Analysis. Exponential Phase. It is important to quantitate your qPCR at the early part of the exponential phase of amplification instead at the later cycles or at the plateau. At the beginning of the exponential phase, all reagents are still in excess.