Tet-On Systems For Doxycycline-inducible Gene Expression
The Tet-Off system allows silencing of gene expression by administration of tetracycline (Tc) or tetracycline-derivatives like doxycycline (dox), whereas the Tet-On system allows activation of gene expression by dox.
Tet systems—Tet-On 3G - Takara Bio
Tet-On 3G systems are inducible expression systems that use a modified Tet transactivator (rtTA3G) to regulate gene expression in response to doxycycline (Dox). They have lower basal expression and higher Dox sensitivity than previous Tet systems.
Introduction to Tet expression systems - The Jackson Laboratory
Tet-On is a binary transgenic system that allows regulated gene expression in mice by doxycycline. Learn how Tet-On works, its advantages and limitations, and how to use it for your research.
『四环素（Tet）调控系统』Tet-on/off 系统—实现基因的诱导表 …
Dec 12, 2021 · 四环素（Tet）调控系统是一种用于诱导基因表达的调控系统，可以根据诱导药物的存在或不存在来控制目的基因的表达。本文介绍了Tet-on/off系统的作用原理和在基因功能和基因治疗领域的应用，以及相关的研究进展和资源。
Tet-On 3G tetracycline-inducible expression systems - Takara Bio
Tet-On 3G is a third generation of powerful and versatile inducible mammalian expression systems that use doxycycline to control gene expression. The system offers low background, high sensitivity, and bidirectional expression of genes of interest and fluorescent markers.
An all-in-one, Tet-On 3G inducible PiggyBac system for human …
May 8, 2017 · The Tet-On 3G system employs a doxycycline-binding transactivator protein and a low background promoter to regulate gene transcription.
Optimization of the Tet-On system for regulated gene ... - Nature
May 25, 2006 · The components of the Tet-On system (the transcriptional activator rtTA and its tetO DNA binding site) were incorporated into the human immunodeficiency virus (HIV)-1 genome to control viral...
Tet-One technology overview - Takara Bio
Tet-One is a single-vector inducible expression system for mammalian cells that can achieve up to 10,000-fold induction with low doses of doxycycline. Learn how Tet-One works, its advantages, and its applications in gene function studies.
A tunable dual-input system for on-demand dynamic gene
Oct 2, 2019 · Here the authors build a Tet-On system with conditional destablising elements to regulate gene expression and protein stability, allowing fine modulation of mESC signalling pathways.
Selecting the optimal Tet-On system for doxycycline-inducible …
The doxycycline (dox)-inducible Tet-On system is widely used to control gene expression in mammalian cells. This system is based on the bacterial Tet operon, which has been modified and improved for its function in eukaryotic cells.
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