Tissue-type plasminogen activator, short name tPA, is a protein that facilitates the breakdown of blood clots. It acts as an enzyme to convert plasminogen into its active form plasmin, the major enzyme responsible for clot breakdown. It is a serine protease (EC 3.4.21.68) found on endothelial cells lining the blood vessels.

Feb 20, 2023 · Assess the mechanism of action of tissue plasminogen activators. Identify the indications for initiating therapy with a tPA agent, differentiating between individual agents where appropriate. Evaluate the contraindications of tPA medications.

Beyond its role on hemostasis, tPA is also a potent modulator of inflammation and is involved in the regulation of several inflammatory diseases. Here, after a brief description of tPA structure, we review its new functions in adaptive immunity focusing on T cells and antigen presenting cells.

(A) Structure of tPA. The 527 amino acids sequence of the protein can be subdivided in five domains that are depicted as follow: 'Finger domain' is in light grey, 'EGFlike domain' is in dark...

t-PA structure and function. Human t-PA is a 68 kDa serine protease composed of 530 or 527 amino acids and containing between seven and 13% carbohydrate. The molecule, is made up of five distinct domain structures with autonomous functions.

Fibrinolysis is dependent on plasminogen and tPA binding via specific domain interactions. There are 5 distinct structural domains in tPA, comprising Finger-Growth factor-Kringle 1-Kringle 2-protease (F-G-K1-K2-P).

May 7, 1998 · Tissue type plasminogen activator (tPA) is the physiological initiator of fibrinolysis, activating plasminogen via highly specific proteolysis; plasmin then degrades fibrin with relatively broad specificity.

Tissue plasminogen activator (tPA) is a drug used to dissolve blood clots. Works by activating plasminogen, which breaks down fibrin in clots. Primarily used for ischemic stroke, heart attack, and pulmonary embolism. Can also be used for certain types of blood clots in other locations.

Full-length tissue-type plasminogen activator (t-PA) cDNA served to construct deletion mutants within the N-terminal "heavy" (H)-chain of the t-PA molecule. The H-chain cDNA consists of an array of structural domains homologous to domains present on other plasma proteins ("finger," "epidermal growth factor," "kringles").

Jan 4, 2024 · The tPA structure is complex and has various functional domains. These domains allow tPA to identify its plasminogen and fibrin substrates and start the fibrinolytic cascade. The specificity with which tPA binds to these substrates is evidence of its crucial function in preserving the delicate equilibrium between clot formation and dissolution ...