SphI - NEB
WEBSphI has a High Fidelity version SphI-HF ® . High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
sphiloilo
WEBSt. Paul’s Hospital of Iloilo, Inc. is located in the heart of the city. It is managed and owned by the Sisters of Saint Paul of Chartres, a tertiary level training general hospital with a capacity of 180 beds and ISO Integrated Management System Certified.
SphI-HF - NEB
WEBProduct Information. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
PaeI (SphI) (10 U/μL) - Thermo Fisher Scientific
WEBThermo Scientific PaeI (SphI) restriction enzyme recognizes GCATG^C sites and cuts best at 37C in B buffer (Isoschizomers: BbuI, SphI). Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction e
Cloning, expression and sequence analysis of the SphI ... - PubMed
WEBNov 21, 1996 · SphI, a type II restriction-modification (R-M) system from the bacterium Streptomyces phaeochromogenes, recognizes the sequence 5'-GCATGC. The SphI methyltransferase (MTase)-encoding gene, sphIM, was cloned into Escherichia coli using MTase selection to isolate the clone.
BspHI - NEB
WEBProduct Information. Product Source. An E. coli strain that carries the BspHI gene from Bacillus species H (D. Hall). This product is related to the following categories: Restriction Endonucleases B Products, Time-Saver Qualified Restriction Enzymes Products. This product can be used in the following applications:
SphI, Restriction Enzymes: "S" Enzymes - Jena Bioscience
WEBAfter 10-fold overdigestion with SphI, >98% of the DNA fragments can be ligated and recut with this enzyme. DNA Methylation: No Inhibition: dam, dcm, CpG. Quality Control: All preparations are assayed for contaminating endonuclease, 3'-exonuclease, 5' exonuclease/ 5' phosphatase, as well as nonspecific single- and doublestranded DNase activities.
Cloning, expression and sequence analysis of the SphI restriction ...
WEBJan 1, 1996 · The nucleotide sequence of the SphI system was determined, analyzed and compared to previously sequenced R-M systems. The sequence was also examined for features which would help explain why sphIR unlike other actinomycete ENase genes seemed to be expressed in E. coli.
RJR REBASE Enz 1719 - SphI
WEBPrototype: SphI Org #: 2481 Organism: Streptomyces phaeochromogenes Organism type: bacteria Organism source: NRRL B-3559 Growth Temperature: 26 ° Experimental Evidence: biochemistry Exhibits star activity Enzyme gene cloned Enzyme gene sequenced Enz Comment: Enzyme is brown and may contain an iron-sulfur cluster (Fe-S). …
Isoschizomer - Wikipedia
WEBIsoschizomer - Wikipedia. Isoschizomers are pairs of restriction enzymes specific to the same recognition sequence. For example, SphI (CGTAC/G) and BbuI (CGTAC/G) are isoschizomers of each other.