Size exclusion chromatography (SEC) is fundamentally different from all other chromatographic techniques in that separation is based on a simple procedure of classifying molecule sizes rather than any type of interaction.

Jun 13, 2020 · Size exclusion chromatography is a technique that separates compounds solely on the basis of size. In order for the results of size exclusion separations to be meaningful, there can be no directed …

Jan 9, 2023 · What is Size Exclusion Chromatography? Size exclusion chromatography is a technique for separating different compounds based on their size (hydrodynamic volume), which is determined by how well they penetrate the pores of the stationary phase.

While numerous techniques have been developed to monitor protein aggregation, size-exclusion chromatography (SEC) has been predominantly favored for routine and validated analyses because of both its speed and reproducibility. [3 – 6] SEC is also an accurate method if confirmed with an orthogonal method, such as sedimentation velocity analytical...

Jan 31, 2025 · Like those resulting from other analytical techniques, a size-exclusion chromatogram is a graph of peaks corresponding to samples eluting at different points in time. Using a calibration graph of standards with known molecular weights, the molecular weight of your sample can be calculated.

Gel Exclusion Chromatography (also called molecular exclusion chromatography, size exclusion chromatography, or gel filtration chromatography) is a low resolution isolation method that employs a cool “trick." This involves the use of beads that have tiny “tunnels" in them that each have a precise size.

separation mechanism is based on the size of the polymer molecules in solution. There are several names given to different types of SEC, but all are based on the same principle, that of size exclusion, hence size exclusion chromatography. Historically the porous medium was made of a gel and therefore gel permeation chromatography was coined, a term

Size exclusion chromatography (SEC) separates molecules based on their size by filtration through a gel. What is Size Exclusion Chromatography?

Ths Size Exclusion Chromatography (SEC) kit is designed to teach basic gel filtration chromatography techniques. This kit utilizes the colored molecules hemoglobin and vitamin B12 to illustrate the principles of SEC. Students can easily visualize the separation of these molecules as they pass through the chromatography column.

ces in size as they pass through a SEC resin packed in a column. SEC resins consist of spherical chromatography particles (chromatography beads) with pores of different sizes where molecules small enough to ente. the pores are retarded as compared to larger molecules (Fig 1.