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Studies of SF3B1 Ulm
National Center for Biotechnology Information
The pre-mRNA splicing and transcription factor Tat-SF1 is a functional partner of the spliceosome SF3b1 subunit via a U2AF homology motif interface
The transcription elongation and pre-mRNA splicing factor Tat-SF1 associates with the U2 small nuclear ribonucleoprotein (snRNP) of the spliceosome. However, the direct binding partner and underlying interactions mediating the Tat-SF1–U2 snRNP association
Author: Sarah Loerch, …
Published: 2019/02/02
Publication: J Biol Che…
PubMed
A UHM-ULM interface with unusual structural features contributes to U2AF2 and SF3B1 association for pre-mRNA splicing
During spliceosome assembly, the 3' splice site is recognized by sequential U2AF2 complexes, first with Splicing Factor 1 (SF1) and second by the SF3B1 subunit of the U2 small nuclear ribonuclear protein particle. The U2AF2-SF1 interface is well characterized,
Author: Justin W Galardi
Published: 2022/8
Keywords: RNA splicin…
ResearchGate
A UHM – ULM interface with unusual structural features contributes to U2AF2 and SF3B1 association for pre-mRNA splicing
During spliceosome assembly, the 3´ splice site is recognized by sequential U2AF2 complexes, first with Splicing Factor 1 (SF1), and second by the SF3B1 subunit of the U2 small nuclear ribonuclear protein particle. The U2AF2-SF1 interface is well-characterized,
Published: 2022/07/01
DOI: 10.1016/j.jbc.2022…
National Center for Biotechnology Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9364107
A UHM–ULM interface with unusual structural features contributes to U2AF2 and SF3B1 association for pre-mRNA splicing
Published: 2022/08
Authors: Justin W. Galardi, Victoria N. Bela, Nazish Jeffer…
DOI: 10.1016/j.jbc.2022.102224
Publication: J Biol Chem. 2022 Aug; 298(8): 102224.
During spliceosome assembly, the 3′ splice site is recognized by sequential U2AF2 complexes, first with Splicing Factor 1 (SF1) and second by the SF3B1 subunit of the U2 small nuclear ribonuclear protein particle. The U2AF2–SF1 interface is well characterized, comprising a U2AF homology motif (UHM) ...
bioRxiv
https://www.biorxiv.org/content/10.1101/2022.01.09.475535v1
A UHM – ULM interface contributes to U2AF2 and SF3B1 ... - bioRxiv
WEBJan 12, 2022 · Using isothermal titration calorimetry, we established similar binding affinities of a minimal U2AF2 UHM – SF3B1 ULM complex and a nearly full-length U2AF2 protein …
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SF3b1
University of Louisiana at Monroe
Cell Press
https://www.cell.com/biophysj/fulltext/S0006-3495(21)01488-0
A UHM-ULM interface contributes to the pre-mRNA splicing …
WEBFeb 11, 2022 · The SF3B1 ULM trajectory across the U2AF2 UHM surface differed from prior structures and would alter the relative orientation of full-length proteins. We showed …
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SF3b1
U2AF2
National Center for Biotechnology Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393619
The pre-mRNA splicing and transcription factor Tat-SF1 is a …
WEBFeb 2, 2019 · We demonstrated that Tat-SF1 preferentially and directly binds the SF3b1 subunit compared with other U2AF ligand motif (ULM)-containing splicing factors, and …
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Publish Year:2019
Presidential Memorial Certificate
Sf3b1 Mutant Cell Lines
bioRxiv
https://www.biorxiv.org/content/10.1101/2022.01.09.475535v1.full.pdf
[PDF]
A UHM – ULM interface contributes to U2AF2 and SF3B1
WEBJan 9, 2022 · Several UHM proteins are known to associate with five ULMs in an intrinsically unstructured, N-terminal region of SF3B1, which additionally contains two …
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SF3b1
University of Louisiana at Monroe
PubMed
https://pubmed.ncbi.nlm.nih.gov/35780835
A UHM-ULM interface with unusual structural features ... - PubMed
WEBWe discovered a distinctive trajectory of the SF3B1 ULM across the U2AF2 UHM surface, which differs from prior UHM/ULM structures and is expected to modulate the …
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University of Louisiana at Monroe
RNA splicing
People also ask
How do SF3B1 ULMS bind other UHMS?
In contrast with the U2AF2 complex, the SF3B1 ULMs bind other UHMs in a curved conformation that stacks a cyclin-dependent kinase–phosphorylation site of SF3B1 against the U2AF2 RXF motif ( 30, 31 ).
A UHM–ULM interface with unusual structural features contributes to
ncbi.nlm.nih.gov
What is SF3B1 ulm5?
SF3B1 ULM5 (residues 333–351) is a relevant, high-affinity ligand for the U2AF2 UHM.A, schematic diagram of the SF3B1 ( yellow )–U2AF2 (navy) complex at the 3′ splice site ( gray ).
A UHM–ULM interface with unusual structural features contributes to
ncbi.nlm.nih.gov
What is the U2AF2 SF3B1 interface?
The U2AF2–SF1 interface is well characterized, comprising a U2AF homology motif (UHM) of U2AF2 bound to a U2AF ligand motif (ULM) of SF1. However, the structure of the U2AF2–SF3B1 interface and its importance for pre-mRNA splicing are unknown.
A UHM–ULM interface with unusual structural features contributes to
ncbi.nlm.nih.gov
How is U2AF2 Uhm SF3B1 ulm5 complex crystallized?
The U2AF2 UHM–SF3B1 ULM5 complex was crystallized by the hanging drop vapor diffusion method at 4 °C from 20.5 mg/ml U2AF2 UHM in the presence of a 1.5-fold molar excess of SF3b155 ULM5 peptide. The reservoir solution contained 0.1 M sodium citrate tribasic dihydrate pH 5.6, 15% v/v 2-propanol, 20% v/v PEG 4000.
A UHM–ULM interface with unusual structural features contributes to
ncbi.nlm.nih.gov
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ResearchGate
https://www.researchgate.net/publication/357781763...
A UHM - ULM interface contributes to U2AF2 and SF3B1 …
WEBJan 9, 2022 · These structures revealed a canonical UHM–ULM interface, comprising a Tat-SF1 binding pocket for a ULM tryptophan (SF3b1 W338) and electrostatic interactions …
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SF3b1
U2AF2
ResearchGate
https://www.researchgate.net/publication/361716795...
(PDF) A UHM – ULM interface with unusual structural features ...
WEBJul 1, 2022 · We discovered a distinctive trajectory of the SF3B1 ULM across the U2AF2 UHM surface, which differs from prior UHM/ULM structures and is expected to modulate …
Tags:
SF3b1
U2AF2
ScienceDirect
https://www.sciencedirect.com/science/article/pii/S0021925820393832
The pre-mRNA splicing and transcription factor Tat-SF1 is a …
WEBFeb 1, 2019 · The SF3b1 ULM region contains >20 TP motifs near the ULMs. These SF3b1 sites are highly phosphorylated (phospho.ELM (40)), which could provide means to …
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Publish Year:2019
Higher-order function
Journal of Biological Chemistry
https://www.jbc.org/article/S0021-9258(20)39383-2/pdf
[PDF]
Thepre-mRNAsplicingandtranscriptionfactorTat-SF1isa ...
WEBSF3b1 ULM interactions are important for association of the proteins in cell extracts. We demonstrate that SF3b1 regulates Tat-SF1 expression levels, and that these splicing …
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