Remel R2A Agar is a solid medium recommended for use in qualitative and quantitative procedures for performing heterotrophic plate counts of treated potable water. SUMMARY AND EXPLANATION.
R2A agar (Reasoner's 2A agar) is a culture medium [1] developed to study bacteria which normally inhabit potable water. [2] These bacteria tend to be slow-growing species and would quickly be suppressed by faster-growing species on a richer culture medium.
R2A Agar is a medium with a low nutrient content, which, in combination with a low incubation temperature and an extended incubation time, is specially suitable for the recovery of stressed and chlorine-tolerant bacteria from drinking water.
As compared to Tryptone Glucose Agar or Standard methods agar, R2A agar has been reported to give improved recovery of stress and chlorine tolerant bacteria from drinking water systems (3,4,5).
R2A Agar was developed by Reasoner and Geldreich for bacterial plate counts of treated potable water. R2A Agar is a low nutrient medium, and in combination with a lower incubation temperature and longer incubation time, stimulates the growth of stressed and chlorine-tolerant bacteria.
Reasoner and Geldreich, of the U.S. Environmental Protection Agency, developed R2A Agar for the recovery and isolation of aerobic and facultative anaerobic heterotrophic bacteria from treated potable water.
Hardy Diagnostics R2A Agar is recommended for enumeration of heterotrophic bacteria in water, especially potable water. R2A Agar is mentioned in the United States Pharmacopoeia, USP <1231> Water for Pharmaceutical Purposes for use in testing hight-purity waters for pharmaceutical purposes.