Phenylthiohydantoin | C9H8N2OS | CID 700731 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.

Trifluoroacetic acid is widely used for this reaction. The thiazolinone amino acid is then extracted into an organic solvent (ethyl acetate or chlorobutane) and converted into more stable phenylthiohydantoin amino acid after treatment with the aqueous acid.

Pehr Edman now also describes the synthesis of phenylthiohydantoin (PTH) derivatives of most of the naturally occurring amino acids; 12 characterizes them by melting point and elementary analysis. With a few exceptions, the chemical stability of PTH-amino acids were excellent.

Phenylthiohydantoin is a chemical compound that is a key intermediate in the Edman degradation, a widely used method for sequencing peptides and proteins. It is formed during the first step of the Edman degradation process, where the N-terminal amino acid of a peptide is selectively cleaved and derivatized.

Jun 18, 2022 · We report a method for the simultaneous determination of the sequence and absolute configuration of peptide amino acids using a combination of Edman degradation and HPLC–MS/CD....

The compound formed from an amino acid in the Edman method of protein degradation, in which phenylisothiocyanate reacts with the amino moiety of the N-terminal amino acid to form a phenylthiocarbamoyl peptide or protein, on which weak acids act to release the phenylthiohydantoin containing the N-terminal amino acid.

In recent years high-pressure liquid chromatography has found increasing application in protein chemistry for the identification of the phenylthiohydantoin derivatives of amino acids. For routine microsequencing none of the solvent systems so far published …

Phenylthiohydantoin-leucine; CAS Number: 4399-40-0; Linear Formula: C13H16N2OS; find Combi-Blocks, Inc.-COM490020824 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich

Protein sequence analysis employing Edman degradation chemistry commonly uses high-performance liquid chromatography (HPLC) separation as the means for identification of the PTH-amino acid (phenylthiohydantoin amino acid) produced at each cycle.

Amino acids, phenylthiohydantoin; Synonyms: PTH-amino acids; find Sigma-Aldrich-PTH26 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich