Phalloidin Conjugates for Actin Staining - Thermo Fisher Scientific
WEBPhalloidin is a bicyclic peptide that belongs to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin in fixed cells, permeabilized cells, and cell-free experiments.Phalloidin conjugates have similar affinity for both large and small …
Structural Effects and Functional Implications of Phalloidin and ...
WEBApr 7, 2020 · Introduction. Actin is a highly conserved and abundant protein which is essential for many key processes, including cell motility, muscle contraction, and vesicle trafficking (Pollard and Cooper, 2009).The 42-kDa protein consists of four subdomains (SDs) enclosing the central metal-nucleotide-binding site, which is occupied by Mg 2+ …
Phalloidin staining protocol | abcam
WEBNov 17, 2021 · Phalloidin is a highly selective bicyclic peptide used for staining actin filaments (also known as F-actin). It binds to all variants of actin filaments in many different species of animals and plants.
Alexa Fluor™ 488 Phalloidin - Thermo Fisher Scientific
WEBAlexa Fluor 488 phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor 488 dye. Selectively stains F-actin
Phalloidin - an overview | ScienceDirect Topics
WEBActin and Actin-Binding Proteins. In Cell Biology (Third Edition), 2017. Actin Filament Stabilizers. Phallotoxins (such as phalloidin), cyclic peptides synthesized by poisonous mushrooms, and the sponge toxin jasplakinolide both stabilize actin filaments.They bind to filaments between three subunits and reduce the rate of subunit dissociation to near …
Structural Effects and Functional Implications of Phalloidin and ...
WEBThe toxins phalloidin and jasplakinolide stabilize actin filaments. Conjugated to different probes they are routinely used for labeling. Despite their popularity, their structural effect on the filament remained elusive. Using cryo-EM, Pospich et al. found the toxins to suspend structural changes in actin, likely influencing the binding of accessory proteins.
WEBCells stained with Biotin‑XX Phalloidin require a fluorescent or enzyme-conjugated avidin or streptavidin detection reagent. Dissolve the vial contents in 0.5 mL of methanol to yield a final concentration of 100 units/assays per mL, which is equivalent to
Fluorescent Phalloidin: A Practical Stain for Visualizing Actin ...
WEBJul 1, 2018 · Phalloidin's ability to target filamentous actin with high specificity makes it a valuable tool in studying actin dynamics and cytoskeletal networks. Enjoy a number of fluorescently labeled phalloidin conjugates to visualize F-actin.... AssayWise Letters 2018, Vol. 7(1). AssayWise Letters is a bi-annual newsletter which discusses recent advances …
Phalloidin - an overview | ScienceDirect Topics
WEBHepatic Toxicology. J.E. Manautou, ... L.M. Aleksunes, in Comprehensive Toxicology, 2010 9.08.3.5.6 Phalloidin. Phalloidin is a member of the group of phallotoxins produced by the mushroom Amanita phalloides, which binds to F-actin and prevents its depolymerization, resulting in cytotoxicity (Wieland 1983).This poison causes cholestatic hepatotoxicity, …
WEBStructure Article Structural Effects and Functional Implications of Phalloidin and Jasplakinolide Binding to Actin Filaments Sabrina Pospich,1 Felipe Merino,1 and Stefan Raunser1,2,* 1Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund 44227, Germany 2Lead Contact *Correspondence: …
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