Escherichia virus T5, sometimes called Bacteriophage T5 is a caudal virus within the family Demerecviridae. This bacteriophage specifically infects E. coli bacterial cells and follows a lytic life cycle.

Mar 24, 2023 · Phage T5 (17), a Siphoviridae infecting Escherichia coli, is a model phage belonging to the T series introduced by Delbrück and co-workers in the 1940s (18).

T5 is a member of the Siphoviridae and is one of the 7 classical Caudovirales or tailed bacteriophages that were originally designated T (for “Type”) phages by Delbrück and co-workers.

T5 is a virulent phage that infects Escherichia coli. We found that CRISPR-Cas9 system (type II-A) had ununiform efficacies against T5, which impairs a reliable use of CRISPR-Cas-assisted counterselection in the gene editing of T5.

Bacteriophage T5 was one of the first enterobacterial phages to be characterized in-depth in the 1950s, and since then several similar bacteriophages have been reported. Their most unique feature is a two-step DNA transfer during infection (reviewed in Davison, 2015).

Aug 13, 2018 · Phage T5 has one receptor-binding protein (RBP) located at the tip of the straight central tail fibre, and three additional L-shaped tail fibres protrude from the baseplate.

Here, we visualize, for the model phage T5, how host recognition and SE can be achieved via the interaction of different phage proteins with the same receptor in the Escherichia coli outer membrane.

The T5 bacteriophage consists of a head, which contains DNA within an ordered protein shell, and a tail component displaying a helical structure with a pitch of 3.9 nm. The challenging task was to image this 3.9 nm pitch by holography.

Bacteriophage T5 is an obligately lytic phage of E. coli that is classified as a member of the Siphoviridae family, having an icosahedral head and a long, noncontractile tail. Its genome is composed of a single linear piece of dsDNA, just over 121,000 base pairs.

Oct 7, 2020 · A new, radically different, mechanism of T5 2-step injection is proposed whereby injecting T5 DNA stops at the plasma membrane due to the binding of the iss DnaA boxes to membrane-bound DnaA protein.