Lipofectamine 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.

Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. The reagent allows for transfection to be performed both in presence or absence of serum.

Dec 5, 2022 · To transfect cells with double stranded siRNA, it is suggested not to add P3000 reagent. However, addition of P3000 component is recommended for DNA samples.

3000 Reagent Protocol. For support visit thermofisher.com/support Transfect cells according to the following table. Use the indicated volume of DNA and . P3000 ™ Reagent with each of the two volumes of Lipofectamine 3000 (when performing optimization). Each reaction mix volume is for one well . and accounts for pipetting variations.

Invitrogen™ Lipofectamine™ 3000 Transfection Reagent is a highly efficient, cost-effective tool for lentiviral production (Figure 1). This versatile reagent enables high viral titers even with genes that are large or difficult to package.

InvitrogenTM LipofectamineTM 3000 Transfection Reagent was developed to unleash the power of stem cells by providing a highly efficient, cost-effective nucleic acid delivery alternative to electroporation.

** Optimum amount needed is determined from the protocol for Lipofectamine 3000 Transfection Reagent. For your convenience, the essential components of this protocol are now available in the Gibco™ Breast Cancer Starter Kit. The kit includes: basal medium, FBS, Lipofectamine 3000 reagent, Opti-MEM medium, and TrypLE reagent.

Lipid-based transfection is based on the electrostatic interaction between DNA-transfection reagent complexes and the cell membrane. Here, reagent P3000 plays a crucial role. After this interaction, complexes enter the cell via endocytosis, leading to the formation of an endosome.

Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.

We recommend using Lipofectamine™ 3000 Reagent or delivery of plasmid DNA, Lipofectamine™ MessengerMAX™ Reagent for delivery of mRNA or short oligos, and Lipofectamine™ RNAiMAX Reagent for delivery of siRNA/miRNA.