Suck It: The Ins and Outs of Mouth Pipetting - Discover Magazine
WEBMar 20, 2013 · With a method that carefully mimics the sucking of a straw, draw a solution upwards through your man-made pipette to your desired volume using the tension created by the reduced air pressure – yes, suction! Maintain the tension with your mouth. Do not suck too hard and inadvertently slurp the solution into your mouth. Careful now.
No more sucking: 60 years of the micropipette
WEBJul 24, 2017 · Scientists make the pipettes themselves by heating fine glass tubes over a flame and pulling them to make a capillary with a constriction, which sets the volume. They are accurate down to 0.001ml, but fragile, hard to keep clean, and require researchers to use their mouths to suck up the samples.
The history and dangers of mouth pipetting | INTEGRA
WEBNov 3, 2023 · Mouth pipetting is an outdated lab technique in which a scientist sucked a liquid into a glass tube with their mouth, similar to using a straw to sip a drink. This method required the user to hold the tube in their lips, dip the tube tip into the liquid in the source container, and aspirate the liquid by inhaling to create a vacuum.
lab techniques - When did mouth pipetting stop becoming a way …
WEBAug 3, 2015 · Almost all modern lab protocols have an addendum prohibiting pipetting by mouth, instead mandating that a Gilson pipette, a rubber pipette ball, or a serological Pipet-Aid be used. However, it was clear that some time in the previous century, prior to the invention of the aforementioned tools, many labs did in fact use mouth pipetting to ...
When Lab Scientists Used to Pipette with Their Mouths - Gizmodo
WEBMar 24, 2014 · That is mouth pipetting. Predictably, pipetting by basically sucking on a straw stuck into your lab samples did, on occasion, yield results that ranged from unpleasant to straight-up poisonings.
Revisiting the Micropipetting Techniques in Biomedical Sciences…
WEBJan 1, 2020 · Pre-rinsing the pipette tips for few times helps to neutralize capillary effects and balances the air temperature inside the tip with that of the sample. Pre-rinsing dramatically improves the accuracy of pipetting, especially very small micro-volumes.
How to Pipette Like a Pro - Labster
WEBMouth-pipetting involves drawing up a solution into a pipette by sucking on it like a straw and transferring it to another vessel. Often single-use glass pipettes had to be individually shaped by hand each time.
Fig. 3, [Mouth pipette production and assembly...]. - Cytoskeleton ...
WEBMouth pipette production and assembly for aspiration of mouse oocytes. (a) A fully assembled mouth pipette containing mouth piece-fitted tubing and a pulled glass micropipette separated with a 0.22 μm filter. (b) The process of cleaning isolated oocytes from debris by washing through nine droplets of M2 + dbcAMP.
How “Mouth Pipetting” Lead to the Invention of the Modern Pipette
WEBJun 13, 2017 · Tested.com have an article on the history of pipettes, or specifically the practice of “mouth pipetting” and how it eventually lead to the invention of the modern lab tool that can measure a zeptoliter of liquid (that’s one billionth of one trillionth of a liter!).
Modern Pipetting - Clinical Lab Products
WEBAug 22, 2018 · This is a companion article to “Pipette Design Takes a Quantum Leap Forward.” Used for diverse applications spanning from analytical research to genetic testing, the pipette is widely considered one of the most crucial instruments found in laboratories.