Learn more about monocytes, including monocyte development, monocyte subset markers, monocyte trafficking and tools to study monocytes.

Apr 11, 2022 · You’ll add an ‘human monocyte isolation cocktail’, consisting of an Fc receptor blocking antibody and combination of monoclonal antibodies that recognise specific cell surface markers to target non-monocytes for removal.

Mar 20, 2025 · Non-classical monocytes (2–11%) patrol the endothelium, contributing to vascular homeostasis and apoptotic cell clearance. Quality and Purity Checks. Ensuring monocyte purity and viability is essential for reliable experimental results. Purity assessments rely on surface marker expression, with flow cytometry being the most precise method.

We aimed to investigate the effects of three commonly used monocyte isolation techniques on the resulting MDM phenotype. Plastic adhesion, negative selection, and CD14 pos selection were compared. Monocyte‐derived macrophages were generated by 5‐day culture with macrophage and granulocyte–macrophage colony‐stimulating factors.

We used Flow cytometry to assess the isolation, purification, and generation of pure monocytes characterized as CD14 +, and expression of mo-DC classical markers (HLA-DR, CD80, CD83, and CD86).

Mar 11, 2025 · Monocytes typically represent 2-10% of circulating white blood cells in humans and play essential roles in inflammation, immune surveillance, and tissue homeostasis. Monocytes originate from hematopoietic stem cells in the bone marrow through a …

In vitro analysis of human macrophages is generally hampered by the necessity to differentiate them from peripheral blood monocytes. We have analyzed to which extent noncancerous SC monocytes could be used as an in vitro macrophage model.

In-vitro model of mononuclear phagocytic activity to predict clinical significance of RBC antibodies.

Notes: Monocyte nuclear and cytoplasmic morphology can be highly variable. 1. Nucleus-to-Cytoplasm Ratio: Variable 2. Nucleoli: Not visible 2. Nucleus:1,2. Variable shapes (Folds, kidney shaped) Loose, lacy, violet chromatin. Cytoplasm:1,2. Blue-gray cytoplasm (Ground glass appearance due to fine, diffuse granules) May have pseudopods.

Monocytes represent an important immune cell population that is important for protecting the host from a wide array of pathogens. HSCs from human cord blood are used to establish a model of human myelopoiesis in order to generate immature monocytes (Fig. 3).