Methyl green has long been known as a histological stain with a specific affinity for DNA, although its fluorescent properties have remained unexplored until recently. In this article, we illustrate the method for preparing a methyl green aqueous stock solution, that when diluted can be used as a ve...

Methyl green has long been known as a histological stain with a specific affinity for DNA, although its fluorescent properties have remained unexplored until recently. In this article, we illustrate the method for preparing a methyl green aqueous stock solution, that when diluted can be used as a ve...

Methyl green (MG) is an inexpensive, nonproprietary, traditional histological stain for cell nuclei. When bound to DNA and upon excitation with orange-red light, it fluoresces brightly in the far red region. We compared MG with ethidium bromide (EtBr), the conventional stain for DNA in gels, and Ser...

MOLECULAR BIOLOGY PRACTICALS. Practical 3. Methyl green-pyronin method for DNA and RNA detection in cells. Compiled by Boris M. Sharga, Diana B. Pylypiv, Volodymir P. Feketa Theoretical background. Methyl green-pyronin (MGP) staining is a classical technique utilizing two basic dyes for the detectio...