Generation of Luciferase-expressing Tumor Cell Lines - PMC
In order to track hematologic tumor cells in an in vivo murine leukemia model, we made the FULGW lentiviral vector that co-expresses firefly luciferase (Luc) and enhanced green fluorescent protein (EGFP) for the purpose of B-cell lymphoma (A20) cell line transduction and …
Other content from pmc.ncbi.nlm.nih.govLuciferase - Wikipedia
Luciferases can be produced in the lab through genetic engineering for a number of purposes. Luciferase genes can be synthesized and inserted into organisms or transfected into cells. As of 2002, mice, silkworms, and potatoes are just a few of the organisms that have already been engineered to produce the protein.
In the luciferase reaction, light is emitted when luciferase acts on the appropriate luciferin substrate. …Wikipedia · Text under CC-BY-SA license- Estimated Reading Time: 11 mins
Luciferase-labeled Cells - ATCC
Luciferase-labeled cell lines provide a simpler and highly sensitive means to measure biological processes and to assess drug efficacy in animal models through bioluminescence imaging. …
Generation of Luciferase-expressing Tumor Cell Lines
Apr 20, 2018 · Methods of introducing bioluminescent genes, such as firefly luciferase, by viral transduction has allowed for the production of tumor cell lines that can be followed in vivo …
- Author: Todd V. Brennan, Liwen Lin, Xiaopei Huang, Yiping Yang
- Publish Year: 2018
Luciferase - an overview | ScienceDirect Topics
Luciferase is an enzyme found in fireflies that facilitates the conversion of luciferin to oxyluciferin in the presence of ATP, O2, and Mg2+, resulting in the emission of yellow light. It is commonly …
KPC-luciferase-expressing cells elicit an anti-tumor immune
Jun 13, 2024 · The expression of foreign proteins, such as luciferase in cancer cells could act as a foreign antigen that is targeted by the immune system, thus altering the adaptive immune …
Novel NanoLuc substrates enable bright two …
Jul 13, 2020 · Antares, a fusion of the luciferase NanoLuc to the orange fluorescent protein CyOFP, has emerged as a bright bioluminescent reporter with orthogonal substrate specificity to firefly luciferase...
GL261 luciferase-expressing cells elicit an anti-tumor immune …
Jul 3, 2020 · GL261 murine glioma cells are widely used as a syngeneic animal model of glioma, however, it has become common practice to transfect these cells with luciferase for …
Bioluminescence Color-Tuning Firefly Luciferases: Engineering and ...
Abstract. Firefly luciferases catalyze the efficient production of yellow-green light under normal physiological conditions, having been extensively used for bioanalytical purposes for over 5 …
Single-cell bioluminescence imaging of deep tissue in …
Feb 23, 2018 · We performed directed evolution on firefly luciferase using a red-shifted and highly deliverable luciferin analog to establish AkaBLI, an all-engineered bioluminescence in vivo imaging system.