poly (I:C) and LPS induce distinct IRF3 and NF-kappaB ... - PubMed
The pathogen-associated molecular pattern, polyinosinic-polycytidylic acid [poly(I:C)], and LPS were used to study type-I IFN and TNF responses in human macrophages. Additionally, activation of the key signaling pathways, IFN-regulatory factor 3 (IRF3) and NF-kappaB, were studied.
Lipopolysaccharide-mediated Interferon Regulatory Factor …
TANK Is Involved in the LPS-mediated IRF3/7-activating Pathway—To demonstrate the existence of an endogenous, signal-responsive TANK-containing protein complex that phosphorylates IRF3/7, we first stimulated RAW 264.7 macrophages with LPS, which is known to activate TBK1-IKKϵ through the TLR4-dependent pathway (23, 41), or with TNFα and ...
STING-IRF3 contributes to lipopolysaccharide-induced cardiac ... - PubMed
In wild type mice and cardiomyocytes, LPS stimulation triggered the perinuclear translocation of STING, which further bound to Type-I interferons (IFN) regulatory factor 3 (IRF3) and phosphorylated IRF3.
IRFs: master regulators of signalling by Toll-like receptors and ...
Sep 1, 2006 · IRF3 and IRF7: activation by ligation of TLR4 and TLR3. The recognition of PAMPs by TLR4 and TLR3 leads to the recruitment of overlapping sets of signalling molecules.
Transcriptional control of metabolism by interferon regulatory …
May 20, 2024 · LPS has been reported to inhibit insulin sensitivity in myocytes, in part by reducing GLUT4 expression 119,120, but it is unknown if IRF3 mediates these effects. IRF3 and hepatic steatosis
IRF3 regulates neuroinflammatory responses and the expression …
Aug 30, 2024 · Here, we show that global IRF3 deficiency delays TLR4-mediated signaling in microglia and attenuates the hallmark features of LPS-induced inflammation such as cytokine release, microglial reactivity, astrocyte activation, myeloid cell infiltration, and …
Inflammation causes insulin resistance in mice via interferon ...
May 30, 2024 · To determine whether IRF3 is required for LPS-induced insulin resistance, we knocked down IRF3 in mature adipocytes using a doxycycline-inducible lentivirus.
Essential role of IRF-3 in lipopolysaccharide-induced ... - PubMed
Jul 11, 2003 · Using IRF-3 deficient mice, we show here that IRF-3 is indeed essential for the LPS-mediated IFN-beta gene induction. Loss of IRF-3 also affects the expression of profile of other cytokine/chemokine genes.
Essential role of IRF-3 in lipopolysaccharide-induced interferon-β …
Jul 11, 2003 · Using IRF-3 deficient mice, we show here that IRF-3 is indeed essential for the LPS-mediated IFN - β gene induction. Loss of IRF-3 also affects the expression of profile of other cytokine/chemokine genes. We also provide evidence that the LPS/TLR4 signaling activates IRF-7 to induce IFN - β, if IRF-7 is induced by IFNs prior to LPS simulation.
Oxidized Low Density Lipoprotein Blocks Lipopolysaccharide …
Jul 2, 2004 · OxLDL pre-treatment of blood-derived macrophages blocks the LPS-induced activation of IRF3, as determined by DNA binding assays and IRF3·CBP co-immunoprecipitations. Macrophages were grown in the presence or absence of OxLDL as described under “Materials and Methods.”
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