The LD50 is usually expressed as the mass of substance administered per unit mass of test subject, typically as
The … See more

As a measure of toxicity, LD50 is somewhat unreliable and results may vary greatly between testing facilities due to factors such as the genetic characteristics of the sample population, animal species tested, environme… See more

Note: Comparing substances (especially drugs) to each other by LD50 can be misleading in many cases due (in part) to differences in effective dose (ED50). Therefore, it is more useful to compare such subst… See more

The LD50 values have a very wide range. The botulinum toxin as the most toxic substance known has an LD50 value of 1 ng/kg, while the most non-toxic substance water has an LD50 value of more than 90 g/kg; a diffe… See more

Animal-rights and animal-welfare groups, such as Animal Rights International, have campaigned against LD50 testing on animals. Several countries, including the UK, have taken steps to ban the oral LD50, and the … See more