The autophagic protein LC3 translocates to the nucleus and …
Sep 16, 2019 · By using biochemical fractionation analysis and imaging techniques, we demonstrate here for the first time the nuclear accumulation of the autophagic marker MAP1LC3/LC3 (microtubule associated protein1 light chain 3)-II, endogenous and exogenously added (AdGFP-LC3, AdtfLC3), in response to cyclic mechanical stress (CMS).
Monitoring and Measuring Autophagy - PMC - National Center for ...
Aug 28, 2017 · Commonly used markers include ULK1, WIPI1 (which is not necessary for autophagy but can be used as a marker), WIPI2, ATG5, and microtubule-associated protein light chain 3 (LC3). All of these ATG factors, but not LC3 homologs, detach before (or immediately after) the completion of the autophagosome.
Following autophagy step by step | BMC Biology | Full Text
Jun 2, 2011 · Most assays for autophagy modulators use the autophagy marker protein LC3 as readout for autophagic activity. LC3 is a mammalian homolog of the yeast ATG8 protein, a ubiquitin-like protein that becomes lipidated and tightly associated with …
LC3/GABARAP family proteins: autophagy-(un)related functions
In this review, we discuss the current knowledge and functions of the LC3/GABARAP family proteins. We focus on processing of the individual family proteins and their role in autophagy initiation, cargo recognition, vesicle closure, and trafficking, a complex and tightly regulated process that requires selective presentation and recruitment of ...
How to interpret LC3 immunoblotting - PubMed
Microtubule-associated protein light chain 3 (LC3) is now widely used to monitor autophagy. One approach is to detect LC3 conversion (LC3-I to LC3-II) by immunoblot analysis because the amount of LC3-II is clearly correlated with the number of autophagosomes.
The LC3 interactome at a glance - PubMed
Jan 1, 2014 · In this Cell Science at a Glance article and the accompanying poster, we present the current LC3 protein interaction network, which has been and continues to be vital for gaining insight into the regulation of autophagy.
Novel Insights into the Cellular Localization and Regulation of the ...
Oct 18, 2020 · The microtubule-associated protein 1A/1B light chain 3B (MAP1LC3B or LC3B) is commonly analyzed as a key marker for autophagosomes and as a proxy for autophagic flux. Three paralogues of the LC3 gene exist in humans: LC3A, LC3B and LC3C.
Beyond autophagy: LC3-associated phagocytosis and endocytosis
Oct 26, 2022 · Overview of select receptors involved in the recognition of phagocytic and endocytic cargoes including apoptotic/dying cells, extracellular pathogens, and protein aggregates, which subsequently activate LC3 lipidation in LAP and LANDO.
LC3-associated phagocytosis at a glance - The Company of …
Feb 20, 2019 · LC3-associated phagocytosis (LAP) is one such novel function for autophagy proteins and is a contributor to immune regulation and inflammatory responses across various cell and tissue types.
LC3 and Autophagy | Springer Nature Experiments
Microtubule-associated protein 1A/1B-light chain 3 (LC3) is a soluble protein with a molecular mass of ∼17 kDa that is distributed ubiquitously in mammalian tissues and cultured cells. During autophagy, autophagosomes engulf cytoplasmic components, including …