iPSC-derived functional human neuromuscular junctions model …
Sep 9, 2019 · The hNMJ is mainly composed of 3 types of cells: MNs, skeletal muscle, and Schwann cells, all of which are differentiated by the temporal expression of MYOD1 in iPSCs.
ASSESSMENT OF IN VITRO HUMAN NEUROMUSCULAR …
In order to better understand human neuromuscular junction (hNMJ) functionality, we developed on-a-chip devices to grow hNMJs on a specific electrode pattern to stimulate pre-synaptic axons and record post-synaptic muscle activity.
Stem cell derived phenotypic human neuromuscular junction …
Bungarotoxin, BOTOX ® and curare dose response curves were generated to demonstrate pharmacological relevance of the phenotypic screening device. This quantifiable functional hNMJ system establishes a platform for generating patient …
Physiology, Neuromuscular Junction - StatPearls - NCBI Bookshelf
May 1, 2023 · The neuromuscular junction (NMJ) is a synaptic connection between the terminal end of a motor nerve and a muscle (skeletal/ smooth/ cardiac). It is the site for the transmission of action potential from nerve to the muscle. It is also a site for many diseases and a site of action for many pharmacological drugs.
Stem cell derived phenotypic human neuromuscular junction …
There are currently no functional neuromuscular junction (hNMJ) systems composed of human cells that could be used for drug evaluations or toxicity testing in vitro. These systems are needed to evaluate NMJs for diseases such as amyotrophic lateral sclerosis, spinal muscular atrophy or other neurodegenerative diseases or injury states.
Bioengineered optogenetic model of human neuromuscular junction
Sep 1, 2021 · Abstract. Functional human tissues engineered from patient-specific induced pluripotent stem cells (hiPSCs) hold great promise for investigating the progression, mechanisms, and treatment of musculoskeletal diseases in a controlled and systematic manner.
Cellular and Molecular Anatomy of the Human Neuromuscular …
The neuromuscular junction (NMJ) plays a fundamental role in transferring information from lower motor neuron to skeletal muscle to generate movement. It is also an experimentally accessible model synapse routinely studied in animal models to explore fundamental aspects of synaptic form and function.
Human neuromuscular junction on micro-structured microfluidic …
Oct 26, 2021 · In order to better understand human neuromuscular junction (hNMJ) functionality, it is important to develop on-a-chip devices with human cells. To investigate this cell network, microfluidic platforms are useful to grow different cell types in isolated compartments.
Bioengineered model of the human motor unit with ... - Nature
Jun 3, 2021 · The neuromuscular junction (NMJ) is the specialised synapse between the post-synaptic skeletal muscle fibre and pre-synaptic terminal of the efferent motor nerve axon. Its...
[PDF] Human neuromuscular junction on micro-structured …
Sep 24, 2021 · This study provides evidence for a physiologically relevant model to mimic a hNMJ that will in the future be a powerful tool, more sensitive than calcium imaging, to better understand and characterize NMJs and their disruption in neurodegenerative diseases.