Apr 23, 2018 · H3K9me3-dependent heterochromatin is a major barrier of cell fate changes that must be reprogrammed after fertilization. However, the molecular details of these events are lacking in early...

Nov 4, 2021 · We examined the tissue-specific expression and accessibility of H3K9me-marked sequences following the loss of MET-2, a SETDB1-like HMT responsible for H3K9me1 and H3K9me2 (refs. 13, 16), and...

We used chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) to analyze the variations in a methylated histone (H3K9me3) in peripheral blood mononuclear cells from 10 MN patients and 10 healthy subjects.

Aug 9, 2023 · To test this hypothesis, we examined the enrichment of lineage-specific H3K9me3 at TF binding sites based on public mESC ChIP-seq data in both ICM and TE of fertilized and SCNT embryos.

Jan 3, 2019 · We discovered high levels of compacted heterochromatin, H3K9me3-decorated, at protein-coding genes in early, uncommitted cells at the germ-layer stage, undergoing profound rearrangements and reduction upon differentiation, concomitant with …

With our optimized protocol called advanced ultra-low-input ChIP-seq (AULiChIP-seq), the input was reduced to 50–100 cells. For validation, we performed AULiChIP-seq for H3K9me3 using 50 and 500 human embryonic stem cells (hESCs), respectively. These data recapitulate results from those generated using conventional ChIP-seq (Figure S1A

To do this, we first analyzed H3K9me3 levels surrounding replication start sites using H3K9me3 ChIP-seq and Repli-seq datasets in HeLa cells from the Encyclopedia of DNA Elements (ENCODE) Project (36,37). The H3K9me3 levels were low at replication start sites compared to adjacent sites (Figure 3A).

Jul 17, 2014 · We performed ChIP-seq for Suv39h1-H/F and Suv39h2-H/F and also profiled H3K9me3 in WT and Suv39h dn ESCs. Quality control of the resulting reads indicated high genomic coverage (a total of 304 M reads) and close correlation between the biological replicates ( Figures S3 A and S3B).

Oct 18, 2021 · Results: We exploit the sets of publicly available data derived from chromatin immunoprecipitation combined with massively parallel DNA sequencing (ChIP-Seq), produced by the The Encyclopedia of DNA Elements (ENCODE) project, to analyze H3K9me3 at assembled satellite DNA repeats in genomes of human cell lines and during mouse fetal development ...

Dec 1, 2022 · The reported dataset is the combined data on ChIP-seq for H3K9ac and H3K9me3 and RNA-seq, which allows tracing the relations between characteristics of H3K9as and H3K9me3 signals, as well as mRNA expression at the level of individual genes, providing a valuable resource for regeneration researchers.