- 475 to 650 nmLearn more:✕This summary was generated using AI based on multiple online sources. To view the original source information, use the "Learn more" links.FITC emits fluorescence from 475 to 650 nm, peaking at 525 nm, which falls in the green spectrum.www.bio-rad-antibodies.com/flow-cytometry-fluores…FITC is a green fluorescein derivative with excitation max. of 498 nm, emission max. of 517 nm and displays a high rate of photobleaching.www.thermofisher.com/us/en/home/life-science/cel…FITC has an excitation maximum of 491 nm which closely matches the 488 nm spectral line found in most instruments, and a relatively broad emission spectrum with a maximum of 516 nm.www.aatbio.com/resources/application-notes/fluore…
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Fluorescein isothiocyanate - Wikipedia
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications [1] [2] including flow cytometry. First described in 1942, [3] FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing …
Fluorescein (FITC) | Thermo Fisher Scientific - US
FITC is a green fluorescein derivative with excitation max. of 498 nm, emission max. of 517 nm and displays a high rate of photobleaching.
Spectrum [FITC (Fluorescein-5-isothiocyanate)] - AAT Bioquest
FITC (Fluorescein-5-isothiocyanate) is a fluorescent compound with an excitation peak at 491 nm and an emission peak at 516 nm. Other spectra of interest include: Cy5 (Cyanine-5) , iFluor …
Fluorescein isothiocyanate (FITC) - AAT Bioquest
FITC (fluorescein isothiocyanate) is widely used to label proteins, antibodies, peptides, hormones, amine-modified oligonucleotides, and other amine-containing molecules with the green fluorescent dye fluorescein. The …
FITC Dye Profile - FluoroFinder
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. …
Wavelength (nanometers) Fluorochromes have unique and characteristic spectra for absorption (excitation) and emission. A single dye is excited at a particular wavelength and emits a …
Fluorescence - Flow Cytometry Guide - Bio-Rad
Light absorbance and light emission of fluorescein isothiocyanate (FITC). A fluorophore's maximal absorbance informs you which laser line is optimal to be used for excitation. In the case of …
How To Read A Fluorescence Spectrum - AAT Bioquest
A fluorescence spectrum is an analytical tool that provides researchers and scientists with data based on the way in which a molecule fluoresces.
Fluorescein Isothiocyanate - an overview | ScienceDirect Topics
Fluorescein isothiocyanate (FITC) has been one of the most common fluorescent labels used to modify proteins and other biomolecules (Chapter 10, Section 1). The isothiocyanate group …
Autofluorescence - Flow Cytometry Guide - Bio-Rad
Most autofluorescence is detected at shorter light wavelengths with most absorbing at 350-500 nm and emitting at 350-550 nm. This is especially true for mammalian cells, which contain many compounds that excite by the 488 nm …