F4/80 as a Major Macrophage Marker: The Case of the …
The F4/80 molecule was established as a unique marker of murine macrophages when a monoclonal antibody was found to recognize an antigen exclusively expressed by these cells.
Identification of macrophages in normal and injured mouse
Mar 16, 2022 · F4/80 expression has been reported in mature macrophage populations 75, but is low or even absent in circulating monocytes 76. Although traditionally considered specific to macrophages, F4/80...
F4/80: the macrophage-specific adhesion-GPCR and its role in ...
The identification of cellular ligands for F4/80 and delineation of its signaling pathway remain elusive but are critical to understand the in vivo role of this macrophage-specific adhesion-GPCR.
CX 3 CR1 differentiates F4/80 low monocytes into pro-inflammatory F4/80 ...
Oct 10, 2018 · F4/80 high macrophages of CX 3 CR1 +/GFP mice displayed a more pro-inflammatory phenotype than CX 3 CR1 GFP/GFP mice (Fig. 7e).
F4/80 as a Major Macrophage Marker: The Case of the ... - Springer
Apr 29, 2017 · The F4/80 monoclonal antibody binds to macrophage populations in H-2 haplotype mouse strains, including ASN (a), C57BL/6 (b), BALB/c (d), DBA/2 (d), CBH (k), CBA (k), and ASW (s), indicating that it does not detect polymorphic human leukocyte antigen (HLA) determinants (Austyn and Gordon 1981).
A Protocol for the Comprehensive Flow Cytometric Analysis of …
Mar 3, 2016 · F4/80 is a widely used marker for monocytes and tissue macrophages. Consistent with this, we find F4/80 to be expressed on all monocytes and macrophages in the tissues we examined. F4/80 is also expressed on small subsets of DC and eosinophils (data not shown).
A function for the macrophage F4/80 molecule in tolerance
The F4/80 monoclonal antibody has been used widely as a marker for mouse macrophages. The antigen (Ag) recognized by the antibody has been characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family, although the function of the molecule has remained elusive.
Frontiers | ADGRE1 (EMR1, F4/80) Is a Rapidly-Evolving Gene …
Sep 30, 2018 · The isolation of F4/80 cDNA revealed that it encodes a large extracellular domain containing multiple Epidermal Growth Factor (EGF)-like calcium-binding domains, linked to a seven-transmembrane domain characteristic of G protein coupled receptors.
Dynamic differentiation of F4/80+ tumor-associated macrophage …
Feb 13, 2023 · F4/80 + TAMs showed proximity to neovascularization and tumor vessels, functionally angiogenic in vivo; and greatly promoted the activation of a few key angiogenic markers such as VEGFA, Ki67,...
EMR1, the human homolog of F4/80, is an eosinophil-specific …
Sep 26, 2007 · The EGF-TM7 F4/80 is a defining marker of murine macrophage populations. Applying flow cytometric analysis using the newly generated mAb A10, and quantitative real-time PCR, we here report the surprising observation that the human ortholog of F4/80, EGF-like module containing mucin-like hormone receptor (EMR)1, is absent on mononuclear ...
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