The application of laser pulses to rapidly lyse individual cells has come about relatively recently. Pulsed laser microbeam-induced cell lysis involves directing a nanosecond pulse from a 532 nm laser through a h… See more

Direct mechanical lysis using sharp surfaces with nanoscale barbs termed ‘nanoknives’ has also been recently … See more

Sonication involves the use of ultrasonic waves to generate localized areas of high pressure resulting in cavitation that can shear apart cells. Sonication has several limitations that keep it from being widely used for sin… See more

Electric fields generating transmembrane potentials of the order of 0.2–1.5 V cause rupture of the lipid bilayer forming pores, and with sufficient magnitudes of electric field strengths and time of exposure lead to cell lysis (T… See more