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  1. ScienceDirect
    https://www.sciencedirect.com/science/article/pii/S0580951704340031

    Live-cell Imaging of Filamentous Fungi Using Vital Fluorescent …

  2. Images of DAPI Fungi

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    See all images
  3. PubMed
    https://pubmed.ncbi.nlm.nih.gov/15488280

    Methods to maximise the staining of fungal propagules with

  4. ScienceDirect
    https://www.sciencedirect.com/science/article/pii/S0167701204002052

    Methods to maximise the staining of fungal propagules with …

    Tags:
    Publish Year:2004
    Staining
  5. Studies of Dapi Fungi
    PubMed
    Methods to maximise the staining of fungal propagules with fluorescent dyes
    The spores and conidia of most fungi have very thick and resistant cell walls that severely impede the staining with fluorescent dyes to allow epifluorescence microscopy to be employed for their direct detection and quantification in natural habitats. In this study, oxidation by sodium hypochlorite ...
    Author: Valeria Prigione
    Published: 2004/12
    Keywords: National In…
    ScienceDirect
    Methods to maximise the staining of fungal propagules with fluorescent dyes
    The spores and conidia of most fungi have very thick and resistant cell walls that severely impede the staining with fluorescent dyes to allow epifluorescence microscopy to be employed for their direct detection and quantification in natural habitats. In this study, oxidation by sodium hypochlorite ...
    Published: 2004/12/01
    Cited By: 0
    Keywords: Microwave…
    PubMed
    Permeabilization method for in-situ investigation of fungal conidia on surfaces
    Aims: 4',6-diamidino-2-phenylindole (DAPI) staining and fluorescent in-situ hybridization (FISH) show great potential for the detection of fungal conidia, also conserving the spatial architecture of their colonization. These investigations are often greatly hampered by the complicated wall structure...
    Author: F Villa
    Published: 2009/2
    Keywords: 4',6-diamidi…
    National Center for Biotechnology Information
    Identification of distinct biological functions for four 3′-5′ RNA polymerases
    The superfamily of 3′-5′ polymerases synthesize RNA in the opposite direction to all other DNA/RNA polymerases, and its members include eukaryotic tRNAHis guanylyltransferase (Thg1), as well as Thg1-like proteins (TLPs) of unknown function that are broadly distributed, with family members in all thr...
    Author: Yicheng Long, …
    Published: 2016/09/09
    Publication: Nucleic A…
  6. Springer
    https://link.springer.com/chapter/10.1007/978-3-030-83749-5_11

    Fluorochrome-Based Methods for Fungal Sample Examination

    Tags:
    Springer Science+Business Media
    Fungus
  7. Nature
    https://www.nature.com/articles/s41598-020-72452-2

    Live-cell imaging with Aspergillus fumigatus-specific fluorescent ...

    Tags:
    Fluorescent Dyes
    Publish Year:2020
    Live cell imaging
  8. PubMed
    https://pubmed.ncbi.nlm.nih.gov/19196441

    Permeabilization method for in-situ investigation of fungal conidia …

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    F. Villa, F. Cappitelli, P. Principi, A. Polo, C. Sorlini
    Publish Year:2009
  9. People also ask
    Can DAPI staining be used to detect fungal conidia?
    Aims: 4',6-diamidino-2-phenylindole (DAPI) staining and fluorescent in-situ hybridization (FISH) show great potential for the detection of fungal conidia, also conserving the spatial architecture of their colonization. These investigations are often greatly hampered by the complicated wall structure of many fungal taxa.

    Permeabilization method for in-situ investigation of fungal

    pubmed.ncbi.nlm.nih.gov
    How long does the tinea pedis fungus live on surfaces?

    Dr. Ilya Aleksandrovskiy

    M.D., MBA · 5 years of exp

    Spores can be found on clothing and shoes, bedding, rugs, and furniture in areas with dead skin cells. Onychomycosis, or toenail fungus, thrives in moist areas such as shoes and boots, and fungal spores can remain alive and active for up to 12 months.
    2 more answers
    bing.com
    Can DAPI staining and fluorescent in-situ hybridization detect fungal conidia?
    Abstract Aims: 4',6-diamidino-2-phenylindole (DAPI) staining and fluorescent in-situ hybridization (FISH) show great potential for the detection of fungal conidia, also conserving the spatial architecture of their colonization.

    Permeabilization method for in-situ investigation of fungal

    pubmed.ncbi.nlm.nih.gov
    What is DAPI used for?
    DAPI is usually used to stained AT-rich regions of DNA within fixed fungal cells. However, it can sometimes be used as a vital probe if the cell is permeable to the dye. Often we find that vegetative hyphae will not take up DAPI whilst germinating spores will.

    Live-cell Imaging of Filamentous Fungi Using Vital Fluorescent Dyes an…

    sciencedirect.com
    Can DAPI be used for confocal microscopy?
    DAPI is normally excited at UV wavelengths which are not very compatible with live-cell imaging. However, it is also excited at 405 nm which is now available for confocal microscopy in the form of a relatively inexpensive blue diode laser.

    Live-cell Imaging of Filamentous Fungi Using Vital Fluorescent Dyes an…

    sciencedirect.com
    How to grow fungi in PDA?
    1. Growth of fungi in PDA at 22 °C in darkness. After sporulation, the conidia suspension is acquired with mili-Q water and scraping and collected with an automatic pipette. 2. 3. After centrifugation, the supernatant should be discarded and the pellet washed with sterile water twice.

    Fluorochrome-Based Methods for Fungal Sample Examination

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  10. ScienceDirect
    https://www.sciencedirect.com/science/article/abs/...

    Live-cell Imaging of Filamentous Fungi Using Vital ... - ScienceDirect

    Tags:
    Publish Year:2004
    Fluorescent Dyes
    Electron Microscopy
    Fungi Cell Image
  11. Oxford Academic
    https://academic.oup.com/lambio/article-abstract/48/2/234/6701368

    Permeabilization method for - Oxford Academic

  12. Springer
    https://link.springer.com/protocol/10.1007/978-1-60761-611-5_20

    Methods to Detect Apoptotic-Like Cell Death in Filamentous Fungi …

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    Springer Science+Business Media
    Publish Year:2010
  13. ResearchGate
    https://www.researchgate.net/figure/DAPI-s…

    DAPI staining of nuclei of the different fungal …

    DAPI staining is shown in magenta to visualize more clearly the fungal nuclei. Immunoreactivity with the fungal antibodies indicated is shown in green. Panels A–G and S: anti- C. glabrata as...

    Tags:
    Staining
    DAPI
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