- DAPI is a fluorescent dye that binds to double-stranded DNA and RNA12. It is used to stain both live and fixed cells2, but it penetrates the cell membrane better in fixed cells2. DAPI can be used to analyze cell cycle1by measuring the amount of DNA in the nucleus. DAPI emits blue light when excited by ultraviolet light12.Learn more:✕This summary was generated using AI based on multiple online sources. To view the original source information, use the "Learn more" links.DAPI is commonly used in cell cycle analyses since preferentially binds dsDNA when cells are permeabilized allowing it to saturate the nucleic acids. DAPI will also bind to dsRNA, but gives emits at a longer wavelength near 500 nm. This method may be combined with surface markers and other intracellular stains.flowcytometry.utoronto.ca/wp-content/uploads/201…As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore provides a marker for membrane viability.en.wikipedia.org/wiki/DAPI
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See all on WikipediaDAPI - Wikipedia
DAPI (pronounced 'DAPPY', /ˈdæpiː/), or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine–thymine-rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, … See more
DAPI was first synthesised in 1971 in the laboratory of Otto Dann as part of a search for drugs to treat trypanosomiasis. Although it was unsuccessful as a drug, further investigation … See more
This DNA fluorescent probe has been effectively modeled using the time-dependent density functional theory, coupled with the IEF … See more
The Hoechst stains are similar to DAPI in that they are also blue-fluorescent DNA stains which are compatible with both live- and fixed-cell … See more
When bound to double-stranded DNA, DAPI has an absorption maximum at a wavelength of 358 nm (ultraviolet) and its emission maximum is at 461 nm (blue). Therefore, for fluorescence microscopy, DAPI is excited with ultraviolet light and is detected through a … See more
DAPI can be used for fixed cell staining. The concentration of DAPI needed for live cell staining is generally very high; it is rarely used for live cells. It is labeled non-toxic in its MSDS and though it was not shown to have mutagenicity to E. coli, it is labelled as a known … See more
Wikipedia text under CC-BY-SA license DAPI (4',6-diamidino-2-phenylindole) - Thermo Fisher …
DAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA. It is excited by the violet (405 nm) laser line and is commonly used as a nuclear …
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DAPI Protocol for Fluorescence Imaging - Thermo …
Step-by-step protocol for the use of DAPI (4′,6-diamidino-2-phenylindole) for nuclear acid (nuclear) staining in fluorescence microscopy.
DAPI Counterstaining Protocols | Thermo Fisher Scientific - US
DAPI is a popular nuclear counterstain for use in multicolor fluorescent techniques. Its blue fluorescence stands out in vivid contrast to green, yellow, or red fluorescent probes of other …
DAPI: a DNA-specific fluorescent probe - PubMed
DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent …
Hoechst & DAPI Staining Protocols – Cell Staining with ... - Biotium
DAPI is stable in dilute solutions, and can be added directly to antifade mounting medium for long-term use. Biotium offers DAPI dilactate, a more soluble DAPI salt, which is useful for making …
Labeling nuclear DNA using DAPI - PubMed
Jan 1, 2011 · Cells must be permeabilized and/or fixed for DAPI to enter the cell and to bind DNA. Fluorescence increases approximately 20-fold when DAPI is bound to double-stranded …
DAPI - an overview | ScienceDirect Topics
Feb 7, 2012 · In fluorescence microscopy, the DNA-binding dye DAPI is routinely used to visualize the intracellular and intramitochondrial distribution of mtDNA in yeast cells, slime …
Cell cycle staging of individual cells by fluorescence …
Jan 29, 2015 · The protocol describes in detail the plating of cells (Step 1) and the fixation and staining of cells with DAPI (Step 2). We outline two fluorescence microscopy protocols to acquire images of...
The use of DAPI fluorescence lifetime imaging for investigating ...
Aug 16, 2016 · DAPI lifetime variations in metaphase chromosome spreads allowed mapping of the differentially compacted regions of chromatin along the length of the chromosomes.
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