Solution 13 - ChemoMetec
Mar 8, 2022 · Solution 13 – AO•DAP is a staining reagent containing acridine orange (AO) and 4’,6-diamidino-2-phenylindole (DAPI). It is used to quantify cell concentration and viability of nucleated animal cell samples.
Other content from chemometec.comApplication Solution 13 - AO•DAPI is used for the measurement of concentrations and viability of nucleated mammalian cells. Principle Solution 13 - AO•DAPI contains two different dyes: AO …
DAPI Protocol for Fluorescence Imaging - Thermo …
A popular nuclear and chromosome counterstain, DAPI (4′,6-diamidino-2-phenylindole) emits blue fluorescence upon binding to AT regions of DNA. This protocol can be used for: Nucleic acid (nuclear) staining in fluorescence …
DAPI (4',6-diamidino-2-phenylindole) - Thermo Fisher …
DAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA. It is excited by the violet (405 nm) laser line and is commonly used as a nuclear …
DAPI - Wikipedia
DAPI (pronounced 'DAPPY', /ˈdæpiː/), or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine–thymine-rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore provid…
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DAPI and Hoechst Nucleic Acid Stains - Thermo Fisher Scientific
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Solution 13 - AO DAPI is used for the measurement of concentrations and viability of nucleated mammalian cells. contains two different dyes: AO staining all nucleated cells and DAPI …
DAPI Counterstaining Protocols | Thermo Fisher Scientific - US
DAPI is a popular nuclear counterstain for use in multicolor fluorescent techniques. Its blue fluorescence stands out in vivid contrast to green, yellow, or red fluorescent probes of other …
BD Pharmingen™ DAPI Solution - BD Biosciences
DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) is a nucleic acid stain that binds to A-T rich regions of DNA along the minor groove. DAPI is predominantly impermeant to live cells, allowing it to be used as a viability dye in unfixed …
DAPI (4 ́, 6-diamidino-2 ́-phenylindole, dihydrochloride) is a dye that fluoresces blue (455 nm) when bound to double-stranded DNA and excited by exposure to 345 nm light.