DAPI - Wikipedia
WEBDAPI (pronounced 'DAPPY', /ˈdæpiː/), or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine – thymine -rich regions in DNA. It is used extensively in fluorescence microscopy.
DAPI (4′,6-diamidino-2-phenylindole) - Thermo Fisher Scientific
WEBDAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA. It is excited by the violet (405 nm) laser line and is commonly used as a nuclear counterstain in fluorescence microscopy, flow cytometry, and chromosome staining.
DAPI: a DNA-specific fluorescent probe - PubMed
WEBDAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids.
DAPI Protocol for Fluorescence Imaging - Thermo Fisher Scientific
WEBStep-by-step protocol for the use of DAPI (4′,6-diamidino-2-phenylindole) for nuclear acid (nuclear) staining in fluorescence microscopy.
DAPI Dye Profile - FluoroFinder
WEBDAPI (4',6-diamidino-2-phenylindole (DAPI) is a blue-emitting fluorogenic DNA-binding dye that can be excited by the 355 nm blue laser and captured with a 450/50 nm bandpass filter. DAPI has an excitation peak at 350 nm and an emission peak at 465 nm.
DNA Labelling Using DAPI: Methodology - News-Medical.net
WEBOct 1, 2018 · DAPI, or 4,6-diamidino2-phenylindole, is a fluorescent nucleic acid dye used to preferentially stain double-stranded DNA. There is some evidence that it preferentially binds AT clusters present...
DAPI Staining - Boster Bio
WEBWhat is DAPI? DAPI, or 4',6-Diamidino-2-Phenylindole, Dihydrochloride, is a commonly used fluorescent dye that binds to double-stranded DNA (dsDNA). What does DAPI stain? DAPI binds to and ‘stains’ double-stranded DNA, preferably binding to A-T-rich regions in DNA. DAPI stain is excited by ultrav.
How does DAPI work? - AAT Bioquest
WEBJul 22, 2020 · DAPI (4?,6-diamidino-2-phenylindole) is a blue DNA binding dye with a preference for A-T rich regions, whose quantum yield is significantly enhanced when bound to double-stranded DNA.
DAPI - Blue Fluorescent DNA Stain - AAT Bioquest
WEBDAPI (4'-6-diamidino-2-phenylindole) is a DNA-specific blue fluorescent dye used in many biological disciplines, including biochemistry, molecular biology, immunology, and microbiology.
DAPI for nucleic acid staining 28718-90-3 - MilliporeSigma
WEBDAPI is used as a nuclear counterstain in our FISH protocol at 2 μg/mL in antifade mounting media. DAPI can also be used as a vital dye to stain mature pollen grains (0.5 μg/mL). DAPI can bind to RNA, but its selectivity for DNA over RNA is high.
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