Taq Polymerase Overview, Function & Uses - Lesson - Study.com
Nov 21, 2023 · Taq polymerase is an enzyme used for DNA amplification in PCR reactions. The reason why taq polymerase is used in PCR is because it helps make copies of DNA by attaching nucleotides to the DNA ...
DNA Replication | Location, Steps & Process - Lesson - Study.com
Nov 21, 2023 · Elongation: DNA polymerase is the replicating enzyme that works in the 5' to 3' direction to create a complementary strand of DNA in the 3' to 5' orientation.
RNA Primer | Definition & Function - Lesson - Study.com
Nov 21, 2023 · An RNA primer is a short segment of single-stranded RNA used as a binding site for DNA polymerase to initiate DNA replication. DNA polymerase is only able to add nucleotides to an existing ...
mRNA Transcription Process & Phases - Lesson - Study.com
Nov 21, 2023 · RNA polymerase is the main enzyme involved in the transcription process. Like DNA polymerase, RNA polymerase also adds new nucleotides to the 3' end of the strand thus, growing the RNA chain in a ...
Lagging Strand of DNA | Definition, Synthesis & Replication
Nov 21, 2023 · DNA polymerase is the enzyme that builds the new DNA strands. It reads the base on the original strand and then adds the corresponding base pair to the new strand. DNA polymerase can only add ...
DNA Helicase | Overview, Definition & Function - Study.com
Nov 21, 2023 · These enzymes include primase, DNA polymerase, and topoisomerase. The two strands of the replication fork run in opposite directions; the strand that runs in the direction of the replication fork ...
Helicase in DNA Replication | Definition & Function
Nov 21, 2023 · Helicase, in DNA replication, breaks the hydrogen bonds that hold the double strand together to expose the single strands, in order to kickstart replication. Helicases form a class of enzymes that ...
DNA Template Strand vs. Coding Strand | Overview & Steps
Nov 21, 2023 · Template DNA and PCR. PCR (polymerase chain reaction) is a technique in molecular biology. It is used to amplify sequences of DNA. It is a powerful tool that can take a few copies of a gene and ...
PCR: Steps Involved in Polymerase Chain Reaction - Study.com
Polymerase chain reaction (PCR) is a laboratory procedure that can create replicas of DNA. Explore the three steps of this revolutionary process: denaturation, annealing, and extension.
DNA Ligase Function, Genetic Engineering & Restriction Enzymes
Nov 21, 2023 · During the replication process, DNA polymerase adds nucleotides to the 3' end of the DNA strand and moves from the 3'-to-5' direction. In the leading strand, DNA is situated in the 3'-to-5 ...