Chromosome conformation capture-on-chip (4C) (also known as circular chromosome conformation capture) captures interactions between one locus and all other genomic loci. It involves a second ligation step, to create self-circularized DNA fragments, which are used to perform inverse PCR .

Apr 23, 2018 · Our work shows that chromatin conformation regulates the dynamic interplay between replication and transcription, unveiling its fundamental role in minimizing conflicts between the machineries...

Jan 10, 2025 · In single cells, variably sized nanoscale chromatin structures are observed, but it is unknown whether these form a cohesive framework that regulates RNA transcription. Here, we demonstrate that the human genome is an emergent, self …

Jan 6, 2025 · In this review, we briefly introduce the molecular approaches, focusing on chromosome conformation capture or “3C” technology and its derivatives, which can be used to probe chromatin folding at resolutions beyond that provided by microscopy techniques.

Jan 10, 2025 · Applied to development and aging, we show the pairing of heterochromatin and transcription at myogenic genes that could be disrupted by nuclear swelling. In sum, packing domains represent a foundation to explore the interactions of chromatin and transcription at the single-cell level in human health.

Jan 31, 2025 · Computational methods offer an alternative approach for characterizing individual chromatin conformations at high resolution. Many existing techniques involve fitting an ensemble of chromatin conformations to the average contact probabilities between each pair of genomic loci (12–17), thereby extracting single-cell information from population genome-wide chromatin conformation capture (Hi-C ...

Here, we discuss new findings, which have linked chromatin conformation with development, differentiation and diseases and hypothesized on various models while integrating all recent findings on how chromatin architecture affects gene …

The dynamics of chromatin conformation involve continuous and reversible changes within the nucleus of a cell, which participate in regulating processes such as gene expression, DNA replication, and damage repair. Here, SEE is introduced, an artificial intelligence (AI) method that utilizes autoenco …

Nov 26, 2024 · The advent of genome-wide chromatin conformation capture (Hi-C) methods has revolutionized our ability to probe the three-dimensional (3D) organization of genomes across diverse species.

Apr 23, 2018 · Our work shows that chromatin conformation regulates the dynamic interplay between replication and transcription, unveiling its fundamental role in minimizing conflicts between the machineries moving along the same DNA template, and …