002014 - B6 CD45.1 Strain Details - The Jackson Laboratory
002014 B6.SJL- Ptprc a Pepc b /BoyJ This C57BL/6 congenic strain is used widely in transplant studies because it carries the differential Ptprc a pan leukocyte marker commonly known as CD45.1 or Ly5.1. Wild-type C57BL/6 inbred mice express the Ptprc b (CD45.2 or Ly5.2) allele.
CD45.1 vs CD45.2 B6 mice more than an isoform difference.
Detailed analysis of CD45.1 and CD45.2 strains, enabled researchers to identify a key difference in expression of natural cytotoxicity receptor 1 (NCR1) on NK cells between the two strains, where CD45.1 mouse strains did not express NCR1.
Cd45.1 小鼠 - 知乎 - 知乎专栏
Jul 26, 2023 · 野生型 C57BL/6 小鼠表达 CD45.2 或 Ly5.2（Ptprc b）的等位基因。 CD45.1 和 CD45.2 之间的编码变异不会影 响 CD45 蛋白功能，但会产生可被单克隆克隆抗体识别的独特表位。
Generation of a C57BL/6J mouse strain expressing the CD45.1
Nov 28, 2023 · The B6 (C57BL/6J) strain, which expresses the CD45.2 isoform, was crossed with the SJL mouse expressing the CD45.1 isoform to introduce the CD45.1 gene on a B6 background.
Pathology Outlines - CD45 (LCA)
Feb 1, 2022 · Ubiquitous cell surface marker of nucleated hematopoietic cells involved in the regulation and activation of the immune system. Detectable by immunohistochemistry or flow cytometry.
CD45.1/CD45.2 Congenic Markers Induce a Selective Bias for CD8
Nov 8, 2023 · CD45.1/CD45.2 congenic markers have been used to track hematopoietic lineage differentiation following hematopoietic stem and progenitor cell (HSPC) transplantation. However, several studies suggest that a bias exists in CD45.1 versus CD45.2 hematopoietic cell reconstitution from HSPCs.
Bone Marrow Transplantation Procedures in Mice to Study Clonal ...
May 5, 2021 · Flow cytometry detection of the CD45 variants using specific monoclonal antibodies enables one to distinguish donor cells (CD45.1 − /CD45.2 +) from competitor cells (CD45.1 + /CD45.2 −) within the blood, and assess the clonal dynamics of the test cells over time.
Single-tube Ptprc SNP genotyping of JAXBoy (CD45.1) and C57BL/6J (CD45 ...
Jun 4, 2024 · SJL-Ptprc a Pepc b /Boy (Ptprc a /CD45.1/Ly5.1) mice. Recently, the Jackson Laboratory directly CRISPR-engineered the Ptprc a allele in C57BL/6J mice. This new isogenic strain, termed JAXBoy, differs from wild-type C57BL/6J mice by two nucleotides, compared to the biologically significant 37 megabase (Mb) SJL interval retained in B6.
Single Targeted Exon Mutation Creates a True Congenic Mouse for ...
Jun 6, 2016 · Here, we define the specific epitope difference between the CD45.1 and CD45.2 alleles and describe the creation and testing of a CD45.1 knockin point mutation in the background of the most commonly used C57BL/6N mouse strain.
CD45 Congenic Bone Marrow Transplantation: Evidence for T …
Dec 23, 2008 · CD45 congenic mice have been used to study stem cell engraftment in the absence of alloreactivity. Recently, impaired engraftment was reported in this model and attributed to weak immune reactivity.
- Some results have been removed