1. Calcein Red-AM is a fluorogenic, cell-permeant fluorescent probe that indicates cellular health by detecting the activity of nonspecific esterases.

    Calcein Red-AM - BioLegend

    www.biolegend.com/en-gb/products/calcein-red-am-18901?GroupID=GROUP2
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  2. People also ask
    Can calcein be used as a Ca 2+ or Mg 2+ indicator?
    Calcein is now rarely used as a Ca 2+ or Mg 2+ indicator because its fluorescence is directly sensitive to these ions only at strongly alkaline pH, and thus it is not particularly useful for measuring Ca 2+ or Mg 2+ in cells.

    Calcein - Wikipedia

    en.wikipedia.org
    Can calcein red be used as a reference dye?
    AAT Bioquest offers Calcein Red™ as a reference dye to Calcein Red™ AM. Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles. Prepare a 2 to 5 mM stock solution of Calcein Red™ AM in high-quality, anhydrous DMSO.

    Calcein Red™ AM | AAT Bioquest

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    How do you use calcein red?
    Add Calcein Red™ AM working solution to the culture. Incubate cells at 37 °C for 30 to 60 minutes. Replace the dye working solution with HHBS or buffer of your choice (containing an anion transporter inhibitor, such as 1 mM probenecid, if applicable) to remove any excess probes.

    Calcein Red™ AM | AAT Bioquest

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    What temperature is calcein?
    Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). ?) Calcein, also known as fluorexon, fluorescein complex, is a fluorescent dye with excitation and emission wavelengths of 495 and 515 nm, respectively, and has the appearance of orange crystals.

    Calcein - Wikipedia

    en.wikipedia.org
    Is calcein AM a multicolor reagent?
    Standard overnight for United States, inquire for international Calcein AM is one of the most popular fluorescent probes used for labeling and monitoring cellular functions of live cells. However, the single color of Calcein AM makes it impossible to use this valuable reagent in the multicolor applications.

    Calcein Red™ AM | AAT Bioquest

    aatbio.com
    What spectral settings are used for calcein analysis?
    Analysis can be performed at an Ex/Em = 490/525 nm in a fluorescent microplate reader or under an appropriate channel in a flow cytometer. Other calceins may require other spectral settings outside of the typical green wavelengths. The measured fluorescence intensity is proportional to the number of viable cells within a sample.

    Experimental Protocol for Calcein AM Assay - AAT Bioquest

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  3. Thermo Fisher Scientific
    https://www.thermofisher.com/order/ca…

    CellTrace™ Calcein Red-Orange, AM - Special Packaging

    CellTrace calcein red-orange AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. Unlike calcein AM (C-1430, C-3099, C-3100), CellTrace calcein red-orange AM is intrinsically fluorescent; …

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  4. Thermo Fisher Scientific
    https://www.thermofisher.com/us/en/home/references...

    Fluorescent Indicators for Zn2+ and Other Metal Ions—Section …

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  6. Wikipedia
    https://en.wikipedia.org/wiki/Calcein

    Calcein - Wikipedia

  7. Thermo Fisher Scientific
    https://www.thermofisher.com/us/en/h…

    Fluorescent Ca2+ Indicators Excited with Visible …

    Invitrogen™ Fura Red™ indicator, a visible light–excitable fura-2 analog, offers unique possibilities for ratiometric measurement of Ca 2+ in single cells by microphotometry, imaging or flow cytometry. The visible-wavelength excitation …

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    Calcium Green 5n
    Fluorescent Calcium Indicators
  8. BioLegend
    https://www.biolegend.com/en-gb/produ…

    Calcein Red-AM - BioLegend

    Calcein Red-AM is a fluorogenic, cell-permeant fluorescent probe that indicates cellular health by detecting the activity of nonspecific esterases. When the acetoxymethyl ester is intact the probe is non-fluorescent, but when it is …

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    Cells
  9. AAT Bioquest
    https://www.aatbio.com/products/calcei…

    Calcein Red™ AM - AAT Bioquest

    Oct 24, 2024 · Non-fluorescent Calcein Red™ AM can easily get into live cells and hydrolyzes to generate strongly fluorescent Calcein Red™ dye. Calcein Red™ dye can be monitored with the common TRITC/Cy3 filter set. AAT …

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  10. FluoroFinder
    https://app.fluorofinder.com/dyes/1611-calcein-red...

    Calcein Red-Orange AM Dye Profile - FluoroFinder

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  11. Wiley Online Library
    https://onlinelibrary.wiley.com/doi/10.1002/…

    Tracking the Endosomal Escape: A Closer Look at …

    In general, it can be concluded that the performance of calcein as an indicator for endosomal escape depends on three main parameters which should be considered when planning a calcein release assay and will be discussed in …

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    Calcein and Related Reporters
    Endosomal Escape
    John Wiley & Sons
  12. AAT Bioquest
    https://www.aatbio.com/resources/applic…

    Experimental Protocol for Calcein AM Assay - AAT …

    Calcein Deep Red™ Principle In live cells, non-fluorescent calcein AM is converted to a fluorescent calcein product after AM ester hydrolysis by intracellular esterases.

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  13. MilliporeSigma
    https://www.sigmaaldrich.com/US/en/product/sigma/c0875

    Calcein Used for the fluorometric determination of calcium and …

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