Bone marrow-derived macrophage - Wikipedia
Bone-marrow-derived macrophage (BMDM) refers to macrophage cells that are generated in a research laboratory from mammalian bone marrow cells. [1] [2] [3] BMDMs can differentiate into mature macrophages in the presence of growth factors and other signaling molecules.
Bone Marrow-derived Macrophage Production - PMC
Here, we describe a procedure to isolate and cultivate macrophages from murine bone marrow that will provide large numbers of macrophages for studying host-pathogen interactions as well as other processes.
Preparation and culture of bone marrow-derived macrophages …
Mar 19, 2021 · The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures.
Mouse Bone Marrow Cell Isolation and Macrophage Differentiation
As such, bone marrow-derived macrophages (BMDMs) function as an essential tool to study the pathogenesis of NASH. BMDMs are primary bone marrow-derived cells which are differentiated into macrophages in vitro in the presence of growth factors.
Bone Marrow-Derived Macrophage - an overview - ScienceDirect
Bone marrow derived macrophages (BMDM) and THP-1 macrophages respond differently to the same stimulus. SIS-ECM and UBM-ECM biologic scaffold materials induce similar but distinct macrophage phenotypes.
Investigation of Macrophage Polarization Using Bone Marrow …
Bone marrow derived macrophages (BMDM) present an ideal in vitro model to understand the mechanisms controlling polarization of activated macrophages 15. Specifically, activation of M1 macrophages can be induced by lipopolysaccharides (LPS) stimulation, while polarization of M2 macrophages can be induced by IL-4 and/or IL-13.
Isolation and culture of murine bone marrow-derived …
Aug 1, 2020 · In this study, we propose a standardised protocol for in vitro BMDM generation and show how the production of NO—as a critical modulator inflammatory status—can vary depending on differentiation strategy employed.
Growing Murine Bone Marrow-Derived Macrophages - PubMed
Bone marrow-derived macrophages (BMDM) are primary macrophages obtained by in vitro differentiation of bone marrow cells in the presence of macrophage colony-stimulating factor (M-CSF or CSF1). They are easy to obtain in high yields, can be stored by freezing, and can be obtained from genetically mo …
Macrophage Exosomes Resolve Atherosclerosis by Regulating Hematopoiesis ...
Jul 7, 2020 · Here, we show that exosomes produced by naive bone marrow-derived macrophages (BMDM-exo) contain anti-inflammatory microRNA-99a/146b/378a that are further increased in exosomes produced by BMDM polarized with IL-4 (BMDM-IL-4-exo).
Bone marrow-derived and peritoneal macrophages have different ...
Oct 13, 2016 · To determine if peritoneal macrophages (PEMs) or bone marrow derived macrophages (BMDMs) resembled aortic macrophages from ApoE−/− mice, their M1/M2 phenotype, inflammatory status, and lipid...