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  1. Nature
    https://www.nature.com/articles/s41589-022-01065-9

    Chemical acylation of an acquired serine suppresses oncogenic

    • All experiments were performed using standard personal protective equipment. All chemical syntheses were performed in ventilated fume hoods operating at a face velocity >90 f.p.m. Handling of Biosafety Le… See more

    Cell Culture

    Ba/F3 cells were a gift from T. Bivona (UCSF) and were maintained in RPMI 1640 (Gibco, … See more

    Nature
    Analysis of GTP-bound Ras by Raf-RBD Pulldown

    Ras•GTP pulldown was performed with glutathione S-transferase-tagged Ras binding domain (GST-RBD) immobilized on agarose beads (cytoskeleton, 10 µl, 30 µg of loaded G… See more

    Nature
    Gel Electrophoresis and Immunoblot

    Unless otherwise noted, SDS–PAGE was run with Novex 12% Bis-Tris gel (Invitrogen) in MES running buffer (Invitrogen) at 200 V for 60 min following the manufa… See more

    Nature
    Preparation of Mouse Stem Cell Virus

    pMSCV-Puro plasmids containing full-length human KRAS genes (wild-type, G12S, G12C) were constructed using standard molecule biology techniques by inserting th… See more

    Nature
    Generation of Stable Ba/F3 Transductants

    One milliliter of MSCV-containing supernatant (vide supra) was added to one well of a six-well plate containing 1 × 106 Ba/F3 cells in 1 ml of media comprised of 60% RMPI … See more

    Nature
    How is S acylation reversed?
    S-Acylation is reversed by deacylation enzymes of the metabolic serine hydrolase superfamily. These enzymes use a conserved catalytic serine for nucleophilic attack on a target electrophile, which in protein S-acylation is the thioester carbonyl group.
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    Table of Contents
    Cell Culture
    Analysis of GTP-bound Ras by Raf-RBD Pulldown
    Gel Electrophoresis and Immunoblot
    Preparation of Mouse Stem Cell Virus
    Generation of Stable Ba/F3 Transductants
    How is S acylation reversed?
     
  1. Studies of Acylation of Serine
    PubMed
    Insights into acylation mechanisms: co-expression of serine carboxypeptidase-like acyltransferases and their non-catalytic companion paralogs
    Serine carboxypeptidase-like acyltransferases (SCPL-ATs) play a vital role in the diversification of plant metabolites. Galloylated flavan-3-ols highly accumulate in tea (Camellia sinensis), grape (Vitis vinifera), and persimmon (Diospyros kaki). To date, the biosynthetic mechanism of these compound...
    Author: Shengbo Yao
    Published: 2022/7
    Keywords: co-expressi…
    National Center for Biotechnology Information
    Insights into acylation mechanisms: co‐expression of serine carboxypeptidase‐like acyltransferases and their non‐catalytic companion paralogs
    Acylation is an important catalytic mechanism that leads to diverse acylated plant secondary metabolites (Cheynier et al., 2013; Gachon et al., 2005; Wilson et al., 2016). Acyltransferases are responsible for the transacylation of natural metabolites. Two enzyme families, BAHD (named according to th...
    Author: Shengbo Yao, …
    Published: 2022/07
    Publication: Plant J. 2…
    National Center for Biotechnology Information
    Insights into the serine protease mechanism from atomic resolution structures of trypsin reaction intermediates
    Atomic resolution structures of trypsin acyl-enzymes and a tetrahedral intermediate analog, along with previously solved structures representing the Michaelis complex, are used to reconstruct events in the catalytic cycle of this classic serine protease. Structural comparisons provide insight into a...
    Author: Evette S. Radi…
    Published: 2006/05/05
    Publication: Proc Natl …
    PubMed
    Understanding the acylation mechanisms of active-site serine penicillin-recognizing proteins: a molecular dynamics simulation study
    Beta-lactam antibiotics inhibit enzymes involved in the last step of peptidoglycan synthesis. These enzymes, also identified as penicillin-binding proteins (PBPs), form a long-lived acyl-enzyme complex with beta-lactams. Antibiotic resistance is mainly due to the production of beta-lactamases, which...
    Author: Mónica Oliva
    Published: 2003/10/01
    Keywords: National In…
    PubMed
    Structure, Function of Serine and Metallo-β-lactamases and their Inhibitors
    Antibiotic resistance in gram-negative bacteria has emerged as a major health threat that occurs because these bacteria actively produce β-lactamases responsible for the inactivation of β-lactam antibiotics. The first β lactamase was reported in E. coli back in 1940, before the release of the first ...
    Author: Parveen Salah…
    Published: 2018
    Keywords: Serine-β-la…
    National Center for Biotechnology Information
    Chemical acylation of an acquired serine suppresses oncogenic signaling of K-Ras(G12S)
    Drugs that directly impede the function of driver oncogenes offer exceptional efficacy and a therapeutic window. The recently approved mutant selective small-molecule cysteine-reactive covalent inhibitor of the G12C mutant of K-Ras, sotorasib, provides a case in point. KRAS is the most frequently mu...
    Author: Ziyang Zhang, …
    Published: 2022
    Publication: Nat Che…
    National Center for Biotechnology Information
    Fatty Acylation of Proteins: The Long and the Short of it
    Long, short and medium chain fatty acids are covalently attached to hundreds of proteins. Each fatty acid confers distinct biochemical properties, enabling fatty acylation to regulate intracellular trafficking, subcellular localization, protein-protein and protein-lipid interactions. Myristate and p...
    Author: Marilyn D. Resh
    Published: 2016/07
    DOI: 10.1016/j.plipres.2…
    PubMed
    Chemical acylation of an acquired serine suppresses oncogenic signaling of K-Ras(G12S)
    Drugs that directly impede the function of driver oncogenes offer exceptional efficacy and a therapeutic window. The recently approved mutant selective small-molecule cysteine-reactive covalent inhibitor of the G12C mutant of K-Ras, sotorasib, provides a case in point. KRAS is the most frequently mu...
    Author: Ziyang Zhang
    Published: 2022/11
    Keywords: National In…
  2. PubMed
    https://pubmed.ncbi.nlm.nih.gov/35437852

    Insights into acylation mechanisms: co-expression of serine

  3. Nature
    https://www.nature.com/articles/s41580-024-00700-8

    Mechanisms and functions of protein S-acylation - Nature

  4. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9541279

    Insights into acylation mechanisms: co‐expression of serine ...

  5. Nature
    https://www.nature.com/articles/s41392-022-01245-y

    Protein acylation: mechanisms, biological functions and …

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  7. ACS Publications
    https://pubs.acs.org/doi/10.1021/ja021369m

    Theoretical Perspectives on the Reaction Mechanism of …

    Sep 4, 2003 · The reaction mechanism of serine proteases (trypsin), which catalyze peptide hydrolysis, is studied theoretically by ab initio QM/MM electronic structure calculations combined with Molecular Dynamics−Free Energy …

  8. Wiley Online Library
    https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/tpj.15782
    [PDF]

    Insights into acylation mechanisms: Co-expression of serine ...

  9. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458980

    Insights into the serine protease mechanism from atomic …

  10. PubMed
    https://pubmed.ncbi.nlm.nih.gov/12945052

    Understanding the acylation mechanisms of active-site serine …

  11. Nature
    https://www.nature.com/scitable/topicpage/enzyme...

    Serine Protease, Enzyme Catalysis | Learn Science at Scitable - Nature

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