14-3-3 Protein, CSF (Prion Disease) - In CSF: Search for the presence of the 14-3-3 protein. The 14-3-3 protein is a marker for some prion diseases, such as Creutzfeldt-Jakob disease (CJD), when a number of other neurodegenerative conditions are excluded.

Our data suggests a very high risk for CJD with 14-3-3 levels above 100,000 AU/mL and a probable diagnosis of CJD based on laboratory parameters above 50,000 AU/mL. Keywords: 14-3-3; Biomarker; Cerebrospinal fluid; Creutzfeldt-Jakob disease.

Assays for CSF 14-3-3 protein are probably moderately accurate in diagnosing sCJD: sensitivity 92% (95% confidence interval [CI] 89.8–93.6), specificity 80% (95% CI 77.4–83.0), likelihood ratio of 4.7, and negative likelihood ratio of 0.10.

14-3-3 proteins were originally discovered as a family of proteins that are highly expressed in the brain. Through interactions with a multitude of binding partners, 14-3-3 proteins impact many aspects of brain function including neural signaling, neuronal development and neuroprotection.

CSF samples of suspect CJD cases seen in the prospective German surveillance study were tested for the presence of 14-3-3 protein by using a modified western blot (WB) technique. WB detected 14-3-3 protein in 95.4% of definite and 92.8% of probable cases.

THE 14-3-3 protein is a normal neuronal protein that is released into cerebrospinal fluid (CSF) in association with acute neuronal injury. 1 It has been suggested that the presence of 14-3-3 protein in CSF is a reliable marker for Creutzfeldt-Jakob disease (CJD), with sensitivity and specificity for this protein reported as high as 96% and 93% ...

Sep 19, 2012 · Assays for CSF 14-3-3 protein are probably moderately accurate in diagnosing sCJD: sensitivity 92% (95% confidence interval [CI] 89.8–93.6), specificity 80% (95% CI 77.4–83.0), likelihood ratio of 4.7, and negative likelihood ratio of 0.10.

Sep 26, 1996 · The immunoassay detected the 14-3-3 protein in cerebrospinal fluid from 68 of the 71 patients with Creutzfeldt–Jakob disease (96 percent; 95 percent confidence interval, 92 to 99 percent).

An increased concentration of 14-3-3 protein in CSF supports the diagnosis of Creutzfeldt Jakob disease (CJD) in a patient who has a compatible clinical illness and neurological examination, characteristic features on EEG and MRI-DWI and in whom other possible causes of rapidly progressive dementia have been excluded.

Aug 12, 2003 · CSF was positive for 14-3-3 protein in three of seven cases with possible CJD (42%). In the group with other diagnoses, three individuals had false-positive results (13%). Their diagnoses were definitive Alzheimer's disease, hypercalcemia, and …

Employing immunoblotting, the researchers identified the presence of 14-3-3 protein in cerebrospinal fluid (CSF), finding a strong association between its presence and CJD.

Aug 11, 2003 · CSF was positive for 14-3-3 protein in three of seven cases with possible CJD (42%). In the group with other diagnoses, three individuals had false-positive results (13%). Their diagnoses were definitive Alzheimer’s disease, hypercalcemia, and …

THE CONCENTRATION in cerebrospinal fluid (CSF) of a constitutive neuronal protein known as 14-3-3 protein has been noted to be increased in patients with Creutzfeldt-Jakob disease (CJD). 1,2 A study in this month's ARCHIVES 3 challenges the clinical utility of using elevated 14-3-3 protein levels as confirmation for diagnosis of sporadic CJD ...

Jun 12, 2001 · Background: Recent reports have suggested that the detection of the 14-3-3 protein in the CSF of patients with Creutzfeldt–Jakob disease is a highly sensitive and specific marker of the disease that might be used as a diagnostic criterion.

Nov 1, 2017 · 14-3-3 is a regulator protein involved in multiple protein kinase signalling pathways, regulation of cell cycles, cytoskeletal structure, transcription and intracellular trafficking and targeting. In the brain, it is expressed in neurons and considered a marker of neuronal damage.

To examine the relationship between presence of 14-3-3 protein in cerebrospinal fluid (CSF) and encephalitis in the SIV/macaque model of HIV CNS disease, CSF levels of 14-3-3 protein were measured by quantitative immunoblotting throughout infection in …

We have created a standard 14-3-3 protein assay to establish the precise diagnostic criteria for the level of the 14-3-3 protein in the CSF of patients with CJD. Our assay uses semi-quantitative analysis of digital images obtained from luminescence on western blots.

Routine analysis of cerebrospinal fluid in CJD is typically normal. Closer examination of CSF however reveals two proteins, designated 130 and 131 as sensitive and specific markers. Recently these protein markers have been identified as 14-3-3 proteins and an immunoassay has been developed.

Jan 31, 2025 · Wakabayashi K, Umahara T, Hirokawa K, Hanyu H, Uchihara T. 14-3-3 protein sigma isoform co-localizes with phosphorylated α-synuclein in Lewy bodies and Lewy neurites in patients with Lewy body disease. Neurosci Lett. 2018;674:171-175.

Mar 1, 2005 · To examine the relationship between presence of 14-3-3 protein in cerebrospinal fluid (CSF) and encephalitis in the SIV/macaque model of HIV CNS disease, CSF levels of 14-3-3 protein were measured by quantitative immunoblotting throughout infection in 6 SIV-infected pigtailed macaques.

Aug 28, 2001 · A positive 14-3-3 assay was the only independent predictor for a shorter time to conversion to clinical definite MS (risk ratio 4.1; 95% CI 1.1 to 15) and to reach an Expanded Disability Status Scale (EDSS) ≥2 at the end of follow-up (odds ratio 14.8; 95% CI 2.86 to 76.8).

4 days ago · It was previously demonstrated that Kif23-iso2 interacts with 14–3–3 in a phospho-S814 dependent manner and that the peptide sequence surrounding phospho-S814 has 14–3–3 binding activity . We could confirm this interaction by immunoprecipitation using tagged versions of Kif23-iso1 and 14–3–3-γ expressed in HEK293T cells ( Fig. 5 ...

4 days ago · Plasma and CSF were collected before and after 4 weeks of treatment from 14 and 13 patients, respectively. Average plasma concentration of montelukast after 4 weeks of treatment was 1621.26 ± 361.11 ng/mL with two outliers around 500 ng/mL. At the same time point, CSF concentration of montelukast was 3.66 ± 1.31 ng/mL.

14-3-3 proteins play a crucial role in the regulation of protein-protein interactions, impacting various cellular processes and disease mechanisms. Recent advancements have led to the development of stabilizers that enhance the binding of 14-3-3 proteins to clients, presenting promising therapeutic …

6 days ago · 14–3–3 proteins play a crucial role in the regulation of protein–protein interactions, impacting various cellular processes and disease mechanisms. Recent advancements have led to the development of stabilizers that enhance the binding of 14–3–3 proteins to clients, presenting promising therapeutic potentials. This perspective provides an updated overview of the latest …