BamHI - Wikipedia
WEBBamHI (pronounced "Bam H one") (from Bacillus amyloliquefaciens) is a type II restriction endonuclease, having the capacity for recognizing short sequences (6 bp) of DNA and specifically cleaving them at a target site. This exhibit focuses on the structure-function relations of BamHI as described by Newman, et al. (1995).
BamHI - NEB
WEBBamHI has a High Fidelity version BamHI-HF ® (NEB #R3136). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.
BamHI (10 U/μL) - Thermo Fisher Scientific
WEBThermo Scientific BamHI restriction enzyme recognizes G^GATCC sites and cuts best at 37°C in its own unique buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.
BamHI-HF - NEB
WEBProduct Information. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
Structure of BamHI Bound to Nonspecific DNA - Cell Press
WEBFeb 10, 2000 · BamHI Forms a Loose, Dynamic Complex with Nonspecific DNA. The noncognate DNA is accommodated loosely within a cleft at the bottom of the BamHI dimer (Figure 1). The DNA protrudes out of the cleft, whereas, in the specific complex, it is almost surrounded by the enzyme (Figure 2).
Regulation of the BamHI restriction-modification system by a …
WEBThe BamHI restriction-modification system contains divergently transcribed endonuclease and methylase genes along with a small open reading frame oriented in the direction of the endonuclease gene. The small open reading frame has been designated bamHIC (for BamHI controlling element).
Structural Biochemistry/Enzyme Catalytic Mechanism/BamHI
WEBAug 19, 2018 · BamHI is a catalysis mechanism that involves three different states. The first state, which is before the reaction occurs, is called the pre-reactive state. Next it's the intermediate state, known as the transition state.
WEBBanmHI is a type II restriction endonuclease (from Bacillus amyloliquefaciens H) that binds as a dimer to the symmetrical sequence 5'-GGATCC-3'[10]. The BamHI recognition sequence differs by only one base pair in each half site from the EcoRI sequence 5' …
1BHM: RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH …
WEBJul 12, 1995 · Structure of Bam HI endonuclease bound to DNA: partial folding and unfolding on DNA binding. The crystal structure of restriction endonuclease Bam HI complexed to DNA has been determined at 2.2 angstrom resolution. The DNA binds in the cleft and retains a B-DNA type of conformation.
Two of A Kind: BamHI and BglII - SpringerLink
WEBBrooks JE, Nathan PD, Landry D, Sznyter LA, Waite-Rees P, Ives CL, Moran LS, Slatko BE, Benner JS (1991) Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts.