A table of fluorophores and their absorbance, emission and visible colors. HEX is a green-light fluorophore with an emission wavelength of 556 nm and an absorbance wavelength of 535 nm.
FITC/FAM Molecular Probes General conjugated label Good 494 nm 518 nm 530DF30 530DF60 eGFP (Green Fluorescent Protein) Clontech Reporter gene assay Poor 488 nm 509 nm 530DF30 520LP HEX ABI General conjugated label Good 530 nm 556 nm 555LP 520LP JOE (6-JOE) ABI General conjugated label Good 520 nm 548 nm 530DF30 520LP
Selecting dyes for multiplex qPCR | IDT - Integrated DNA Technologies
Jun 18, 2020 — FAM is a common dye used in multiplex quantitative PCR (qPCR) to identify low-copy transcripts. Learn how to choose the best dye combinations for your instrument and assay with the IDT PrimeTime Multiplex Dye Selection Tool.
What is FAM fluorescence? - SyronOptics
FAM fluorescence is the green emission of a dye called Fluorescein Amidite (FAM), which is used as a label or reporter in DNA sequencing, qPCR, and fluorescence microscopy. Learn about the properties, applications, and advantages of FAM fluorescence in this technical article.
FAM/HEX Dual Channel Calibrator Set | Chai
The FAM + HEX Calibrator Set contains everything necessary to calibrate the Open qPCR dual channel instrument. The calibration process compensates for well-to-well variations to ensure accuracy and reliability in the amplification results.
single tube, with the combination of either FAM and HEX, Cy5 and Cy5.5, or ROX and ATTO 590 targeting the mutant and wild-type alleles, respectively. Ordering Information Visit bio-rad.com/digital-assays to order ddPCR Mutation Detection Assays. Storage and Stability ddPCR Mutation Detection Assays are stable for 12 months
Mutant target and wild-type assays are mixed together and provided in a single tube, with FAM targeting the mutant allele and HEX targeting the wild-type allele. Restriction Enzyme Digestion of Sample DNA.
Quantitative PCR and Digital PCR Detection Methods
Learn about different detection methods for qPCR and dPCR, such as dsDNA-binding dyes, probes, quenchers and nucleic acid analogs. Find out how to use SYBR Green I, Dual-Labeled Probes, HEX and other dyes for fluorescence measurement.
The FAM + HEX Calibrator Set contains all the necessary reagents to run calibration on the Open qPCR instrument quickly and efficiently. It is important to select dyes that are compatible and can be calibrated with the thermal cycler.
Optimization of digital droplet polymerase chain reaction for ...
Jan 7, 2016 — The droplet reader used (QX100 system) is able to discriminate between signals from two channels: FAM and HEX/VIC. In our experience droplet separation in the FAM channel was noticeably better than in the HEX/VIC channel.