Nature4mon
Sanger sequencing’s data problem – and how to solve it
Sanger sequencing is the unsung workhorse of molecular biology, one whose value has long been undermined by the tedious nature of processing its data. Converting files of raw sequencing data into ...
Nature4y
DNA sequencing
DNA sequencing is a laboratory method used to determine the sequence of a DNA molecule. The method was developed by Frederick Sanger in 1975, who was later awarded the Nobel Prize in chemistry in ...
unr.edu5y
Sanger sequencing: Optimal amount of template and primer
At the Nevada Genomics Center we offer DNA sequencing using dye-terminator Sanger sequencing with analysis on an Applied Biosystems SeqStudio Genetic Analyzer. A Sanger sequencing reaction is run with ...
The Scientist7y
Q&A: Confirming Next-Gen Sequencing Results with Sanger
“There is significant debate within the diagnostics community regarding the necessity of confirming NGS variant calls by Sanger sequencing, considering that numerous laboratories report having 100% ...