Various types of endonucleases – enzymes that can cut DNA – were already known before CRISPR-Cas9. The discovery of restriction enzymes in the early 1970s heralded a new age in molecular biology.

But in practice with the help of a battery of kits, all you need to know is the nature of the ends of your fragments—staggered, blunt and perhaps containing sites for restriction enzymes.

CRISPR/Cas-initiated HR in mouse embryos will be used to generate founder animals harboring inserted sequence targeted to the ROSA26 locus. The targeting will be done in embryos using microinjection ...

Researchers might just have come across an Enzyme that can slow ageing. They concluded that fat byproducts such as fatty acids and glycerol contribute to lower life span and more chronic diseases. By ...

Now, practitioners of calorie restriction are hoping that human beings can also drink from the fountain of youth. While only time will tell if it actually works, experts and believers weigh in on ...

A temporary restriction zone brought in to help stem the spread of the bluetongue virus has been replaced by a larger exclusion zone. After confirmation of the virus, which affects some species of ...

A popular experiment that's recommended to demonstrate the functionality of enzymes is chewing a piece of bread for a moment, then paying attention to when it turns from a starchy flavor to begin ...

Plus, the crowds are lighter, although the temperature will be cooler. Most of Africa is restriction-free for fully vaccinated travelers, although a handful of nations require a pre-arrival test.

How does your body speed up these important reactions? The answer is enzymes. Enzymes in our bodies are catalysts that speed up reactions by helping to lower the activation energy needed to start a ...

In a recent study, researchers explored the use of seawater as a retting agent for banana pseudo-stems, achieving an efficient extraction of natural fibers while conserving freshwater resources ...